Any one tell me what theses spots are from

Not to me; Ca def usually presents as punctuate orange-brown lesions. To me that looks like injury, perhaps from bugs or mites.
Spell check, sigh “punctate”
I’ve thoroughly checked for critters couldn’t see any at all growing under led so maybe ca def
 
You don't have to nuke them. But your entire environment is contaminated. Sterilize it. Or they will be back.
 
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