per the directions from my 25 site cloner machine. rinse out all old water, add 1 cap full of bleach, refill to normal level. run for 10 mins. dump out and rinse again. refill to normal level, add 1 capful of hydrogen peroxide. run for 10 mins. dump out. refill to normal level with water only. run for 10 mins. dump out. done. run this with pump and sprayers for all steps.