cloning a cloned clone

aeviaanah

aeviaanah

Well-Known Member
what happens when you keep cloning a clone then cloning off that clone and so on? i think this hurts genetics of the plant. can someone explain to me what happens when one does this?
 
doitinthewoods

doitinthewoods

Well-Known Member
aeviaanah said:
what happens when you keep cloning a clone then cloning off that clone and so on? i think this hurts genetics of the plant. can someone explain to me what happens when one does this?
Click to expand...
You start to get a genetic drift, and it becomes a different plant all together, usually of lesser quality.
 
tom__420

tom__420

Well-Known Member
https://www.rollitup.org/1201734-post1.html

No Cross-Posting
Do not post the same discussion more than once on a discussion forum or on many forums. Duplicate discussions are frustrating and counterproductive for other members, especially for those whose time and energy is limited. Duplicate discussions will be deleted.
 
tom__420

tom__420

Well-Known Member
Not trying to question you man but I have never heard of marijuana's genetics changing under any circumstances. What would make that happen when doing deep tissue culture?
 
moash

moash

New Member
tom__420 said:
Not trying to question you man but I have never heard of marijuana's genetics changing under any circumstances. What would make that happen when doing deep water culture?
Click to expand...
deep tissue culture
ill try to find the link i found while researching and post it
 
moash

moash

New Member
ok so i guess cutting wont suffer from somaclonal variation but DEEP tissue culture can suffer this from the incorrect hormone levels used.....just a thought
SOMACLONAL VARIATION

Leonard N. Bloksberg bloksber at pilot.msu.edu
Tue Sep 26 18:38:00 EST 1995

.>.Somaclonal variation: Somatic mutations which are clonaly propagated. .Mutations happen all the time. Every scheme for isolating insertion mutants(transposon, T-DNA, etc) is plagued by random mutations. For more info aboutsomaclonal mutation, try looking up some of the early work by Evans and Sharp. In a nutshell, the frequency of somatic mutation in tissue culture can be increased or decreased by certain practices. As I recall, higher auxinlevels and longer time spent in the de-differentiated state both tend to increase mutation rates. I am not aware of any evidence that the rate of somatic mutation in a minimised protocol is any different than the normalrate. In personal discussions with Dr. Evans, he suggested that the rate ofmutation in his minimised system is not statistically different from the non-tissue culture rate of mutation (or so it appeared to him in preliminary observations at that time). At that time he was focussing most of his effort on maximising mutation rates to increase variation for selection in breeding programs. Bottom line, minimise exposure of your plants to known mutagens. Random mutations are likely to be an issue in any tagging experiment, finda way to weed them out quickly. Good luck..Leonard N. Bloksbergbloksber at pilot.msu.edu..
 
jwop

jwop

Well-Known Member
moash said:
ok so i guess cutting wont suffer from somaclonal variation but DEEP tissue culture can suffer this from the incorrect hormone levels used
SOMACLONAL VARIATION

Leonard N. Bloksberg bloksber at pilot.msu.edu
Tue Sep 26 18:38:00 EST 1995

.>.Somaclonal variation: Somatic mutations which are clonaly propagated. .Mutations happen all the time. Every scheme for isolating insertion mutants(transposon, T-DNA, etc) is plagued by random mutations. For more info aboutsomaclonal mutation, try looking up some of the early work by Evans and Sharp. In a nutshell, the frequency of somatic mutation in tissue culture can be increased or decreased by certain practices. As I recall, higher auxinlevels and longer time spent in the de-differentiated state both tend to increase mutation rates. I am not aware of any evidence that the rate of somatic mutation in a minimised protocol is any different than the normalrate. In personal discussions with Dr. Evans, he suggested that the rate ofmutation in his minimised system is not statistically different from the non-tissue culture rate of mutation (or so it appeared to him in preliminary observations at that time). At that time he was focussing most of his effort on maximising mutation rates to increase variation for selection in breeding programs. Bottom line, minimise exposure of your plants to known mutagens. Random mutations are likely to be an issue in any tagging experiment, finda way to weed them out quickly. Good luck..Leonard N. Bloksbergbloksber at pilot.msu.edu..
Click to expand...
thanks for posting that!

let me know when you find the bottom of the deep tissue culture pool!!

stay in school...
 
tom__420

tom__420

Well-Known Member
So why on earth would you choose a process to reproduce plants if there was a risk of genetic mutation. Stick to cutting clones, this isn't microbiology
 
You must log in or register to reply here.
Top