Cloning Plants By Tissue Culture

WolfZen

WolfZen

Member
pharmacoping said:
yep, especially if you weren't paying attention in class, tissue culture 101. Then, we eat the bio luminescent material for the glow in the dark poop silly, what else ? nitro might have some ideas, but I'm too busy to play.
Click to expand...
That's the most constructive thing you could do with it. There is nothing else you could do without a lot more knowledge and equipment than you have access to - so why are you asking me 'what else' like it's obvious? You're presenting yourself as someone doing something with genetics, so why not discuss something specific about how you plan on making use of lux genes taken from the bacteria. How are you going to isolate those genes? How are you going to copy them? PCR? What kind of PCR equipment do you have? How are you going to vector them into a plant if successful? How would you get the theoretical plant to then express LuxR?
 
pharmacoping

pharmacoping

Active Member
WolfZen said:
That's the most constructive thing you could do with it. There is nothing else you could do without a lot more knowledge and equipment than you have access to - so why are you asking me 'what else' like it's obvious? You're presenting yourself as someone doing something with genetics, so why not discuss something specific about how you plan on making use of lux genes taken from the bacteria. How are you going to isolate those genes? How are you going to copy them? PCR? What kind of PCR equipment do you have? How are you going to vector them into a plant if successful? How would you get the theoretical plant to then express LuxR?
Click to expand...

Get back to your "computer monitor clone warmer" or your xbox,or whatever you kids do while dreaming of producing marijuana for the masses.
are you culturing? please share some experiences, like wacky flowering times, or homemade additives, or something. nobody has time to cater to your negativity in this forum, until they get out of school, maybe at 3pm. everyone has read your posts thusfar, and must say, not so impressed by your information. . If you're so sure about these foundations of yours, why are you here taunting peolple with real life experiences.
If you are genuinely interested in my bio lumi mj plant, PLACE A BID NOW, public or private.
I will not give you these specific answers, as I might be here just to sell something, remember?

fyi, everyday, when each of us clones,or cultures, we are genetically modifying plant material, albeit near impossible on an Xbox warming plate, or a desk light for illumination.

PICS or GTFO wolfzen, really. we dont read words well, so if you feel the need to type more, just dont! and for the record, I Do think your mom is hot !
 
pharmacoping

pharmacoping

Active Member
nitro20% said:
Dust and mold free??? isnt that the reasoning for all the bleaching and alcoholing before jar???
Click to expand...
right, but heres some food for thought. a dusty, dirty, moldy room/plant is much more likely to carry ???? who knows what to the jar. may not even report as mold? might just die, or mutate or? so its just a good measure.
I'm way less technical than many posters here. maybe an outside of the box look is why I'm able to actually perform this with success, and the smarty pants are afraid to even read the one book that puts an end to their fears, or even mix some agar !

if it was read, me, you, and bonkleesha would be the only ones here, until they assembled their lab !
 
pharmacoping

pharmacoping

Active Member
Plants From Test Tubes-name of the most comprehensive do it yourself culture book ever written

One person here, thinking outside the box, without any admitted biology experience, fearless, and determined, in less than a month, has success in tissue cultured marijuana, with off the shelf supplies. Need I say more ?

Congratulations Nitro20%
 
WolfZen

WolfZen

Member
pharmacoping said:
Get back to your "computer monitor clone warmer" or your xbox,or whatever you kids do while dreaming of producing marijuana for the masses.
are you culturing? please share some experiences, like wacky flowering times, or homemade additives, or something. nobody has time to cater to your negativity in this forum, until they get out of school, maybe at 3pm. everyone has read your posts thusfar, and must say, not so impressed by your information. . If you're so sure about these foundations of yours, why are you here taunting peolple with real life experiences.
If you are genuinely interested in my bio lumi mj plant, PLACE A BID NOW, public or private.
I will not give you these specific answers, as I might be here just to sell something, remember?

fyi, everyday, when each of us clones,or cultures, we are genetically modifying plant material, albeit near impossible on an Xbox warming plate, or a desk light for illumination.

PICS or GTFO wolfzen, really. we dont read words well, so if you feel the need to type more, just dont! and for the record, I Do think your mom is hot !
Click to expand...
Why are you so upset by any specific questions about what you are trying to do?
 
Bonkleesha

Bonkleesha

Active Member
WolfZen said:
That's the most constructive thing you could do with it. There is nothing else you could do without a lot more knowledge and equipment than you have access to - so why are you asking me 'what else' like it's obvious? You're presenting yourself as someone doing something with genetics, so why not discuss something specific about how you plan on making use of lux genes taken from the bacteria. How are you going to isolate those genes? How are you going to copy them? PCR? What kind of PCR equipment do you have? How are you going to vector them into a plant if successful? How would you get the theoretical plant to then express LuxR?
Click to expand...
not genetics. plant tissue culture.
 
WolfZen

WolfZen

Member
Google what information? You won't even state what it is you are trying to do. First it's something about discovering genes and bioluminescing bacteria, then it's nothing to do with genetics. Then what are you talking about? Connect the dots and make some sense by following through with your thoughts.

And again, is it really so hard for you to answer any questions without getting upset and being insulting please?
 
pharmacoping

pharmacoping

Active Member
check these out;

http://www.makeitbreak.com/bioconst-demo/
http://www.sciencedirect.com/science/article/pii/S0168945202000444

Creeping bentgrass (Agrostis palustris Huds.) is a cool season grass widely used on putting greens in golf courses. Transformation of creeping bentgrass has been conducted using microprojectile bombardment and protoplast electroporation. The objective of our study is to develop an alternative and more efficient approach in transforming the grass using Agrobacterium (strain EHA 101). This technique was effective in transforming 40-day old calli derived from mature seeds cultured on MS medium supplemented with 2,4-D, kinetin, and sucrose. Dozens of transgenic plants have been produced from two independent transformed calli. Presence of functional green fluorescence protein (GFP) was detected in leaves, stems, and roots of transgenic seedlings. Four putative transgenic plants and two control plants were randomly chosen and analyzed by Southern blot analysis. Bands corresponding to the GFP gene were clearly shown in transgenic plants. These results indicated that Agrobacterium transformation can successfully be applied to creeping bentgrass.

http://aob.oxfordjournals.org/content/85/6/831.full.pdf
http://www.biomedcentral.com/1471-2229/10/165
 
pharmacoping

pharmacoping

Active Member
no, I wont connect the dots for you. you're not a tissue culturist, you're an antagonist. you were very insulting in the beginning of this thread. I dont want to answer your questions because I do not like your tone.

peace
 
pharmacoping

pharmacoping

Active Member
imagine the impossibilities. like plants that lite up when they need something, maybe different colors for diff needs? christmas trees/no lights, lumi trichomes, wow, I'm so bummed it's only sci fi, in your closet
 
You must log in or register to reply here.
Top