A few plants here are near their 20th generation clone.
If a plant gets fucked up along the way, like it starts flowering then revegged, then it can be effected for life.
My grapefruit x blueberry starts to flower when badly rootbound, even under 20 hrs of light. It recently got so bad that after I transplanted it, it showed all the signs of reveg. Flowering a little of it now, and it is just not the same. I suspect it never will be.
So say you take 15 clones from your mother and then throw her out. You use 14 clones for your flower and then you turn one into your mother. When you do this, use the healthiest clone. You do not want the genetics to be passed on through a weak clone.
My point is, the number of times you take a cutting to make a new mum will have little to no effect. But, little mishaps over several years will add up.