BEST TOP NEWS STORY ~Mosaic Virus in Cannabis~

hexthat

Well-Known Member
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"this took a long time to do.....hope some of you appreciate it



It appears some of you are in the dark or unable to grasp the concept that this Mosaic virus which is kicking many asses out there in the cannabis realm is real!


Doing some research into the fact that this was the first virus ever discovered in the 19th century and yes it's still alive and kicking today,,,being spread around from grow to grow simply because so many numbskulls want to remain stupid!


It is easy to distinguish from ph swings and nute deficiencies and insect damage, but yet many continue to breed sick plants and spread the disease to others via seed stock,or infected cuts..


Failure to learn the symptoms will result in a widespread and wholesale spread of this nasty ass disease...


All this was copied and pasted from a few different links, hope you enjoy.



Here is some details about it for starters..


Tobacco Mosaic Virus

Tobacco mosaic virus (TMV) has been at the center of virus research since its discovery over a hundred years ago. TMV was the first virus to be discovered. Late in the 19th century, researchers found that a tiny infectious agent, too small to be a bacterium, was the cause of a disease of tobacco plants. It then took 30 years of work before the nature of this mysterious agent became apparent. In a Nobel-prize-winning study, Wendell Stanley coaxed the virus to form crystals, and discovered that it was composed primarily of protein. Others quickly discovered that there was also RNA in the virus. Then, many prominent structural researchers (including J. D. Bernal, Rosalind Franklin, Ken Holmes, Aaron Klug, Don Caspar, and Gerald Stubbs) used X-ray diffraction and electron microscopy to probe the structure of the virus.
A Helical Virus

Several structures of the whole tobacco mosaic virus are available in the PDB, including the ground-breaking one solved by X-ray fiber diffraction (shown here from PDB entry 2tmv), and a recent structure solved by analysis of many electron micrographs (PDB entry 2om3). The virus is composed of one strand of RNA (shown in red) wrapped inside a sheath of protein (shown in blue). The protein coat is composed of about 2130 copies of a small protein, which stack like bricks in a cylindrical chimney. The RNA strand encodes four proteins, which together orchestrate the life cycle of the virus. These include two proteins that replicate the viral RNA, a protein that transports the RNA from cell to cell, spreading the infection, and the capsid protein seen in the PDB structures.
TMV Infection

Tobacco mosaic virus is very stable, so stable that it can survive for years in cigars and cigarettes made from infected leaves. The viral RNA is infectious by itself, but the addition of a protein coat protects the RNA from enzymes that would destroy it. The protein coat poses a problem, however: it must be removed once the virus gets inside a cell. TMV uses two tricks to release its RNA. As with many viruses, TMV has a chemical switch that causes the protein to change when the environment changes. The capsid protein has several clusters of acidic amino acids that are stable outside of cells, where calcium levels are high, but repel one another in the low-calcium conditions inside cells. This is enough to loosen the first few capsid proteins, releasing the end of the RNA. TMV then uses ribosomes as the engines to finish the job. As the ribosomes move down the strand, creating the first set of virus proteins, they displace the remaining capsid proteins.



Many times it will appear the plant is healthy to the untrained eye..." - forum.grasscity.com

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I'm still in shock as to how any idiots are here in disbelief.
 

hexthat

Well-Known Member
This virus is cross species, the tomato infected with the strain can give it to okra plant, smoke from tobacco products is the #1 cause of infection.
 

hexthat

Well-Known Member
Has anyone had a test show positive for TMV from a cannabis sample?
no clue but if you pay for it i can get you a few samples off different strains

"This ELISA test is a qualitative seriological assay for detection of Tobacco mosaic virus (TMV) in crops such as vegetable, fruit, and ornamentals. Tobacco mosaic virus is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.
This test detects a variety of viruses from the tobamovirus group including Tobacco mosaic virus-common strain (TMV-c), Tomato mosaic virus (ToMV), and Sunn hemp mosaic virus (SHMV). This broadly reacting TMV assay is recommended whenever you wish to test for the presence of TMV without needing to distinguish TMV-c from other tobamoviruses. This test, however, is not a tobamovirus group test. For example, it does not react with Ribgrass mosaic virus." - https://orders.agdia.com/InventoryD.asp?loc=IN&collection=PSA 57400&attribute_Size=288
 

hexthat

Well-Known Member
"General Information about Tobacco Mosaic Virus : When Tobacco Mosaic Virus infects a tobacco plant, the virus enters mechanically (For example through a ruptured plant cell wall) and replicates. After its multiplication, it enters the neighboring cells through plasmodesmata. For its smooth entry, Tobacco Mosaic Virus produces a 30,000 dalton protein called P30 which tends to enlarge the plasmodesmata. TMV most likely moves from cell-to-cell as a complex of the RNA, P30, and replicase proteins.

The first symptom of this virus disease is a light green coloration between the veins of young leaves. This is followed quickly by the development of a “mosaic” or mottled pattern of light and dark green areas in the leaves. These symptoms develop quickly and are more pronounced on younger leaves. Mosaic does not result in plant death, but if infection occurs early in the season, plants are stunted. Lower leaves are subjected to “mosaic burn” especially during periods of hot and dry weather. In these cases, large dead areas develop in the leaves.

This constitutes one of the most destructive phases of tobacco mosaic virus infection. Infected leaves may be crinkled, puckered, or enlongated.
Consumption of tobacco products infected with the Tobacco Mosaic Virus has been found to have no effect on humans.[citation needed]

This plant disease can commonly be found in these places:
The plant disease caused by tobacco mosaic virus is found worldwide. The virus is known to infect more than 150 types of herbaceous, dicotyledonous plants including many vegetables, flowers, and weeds. Infection by tobacco mosaic virus causes serious losses on several crops including tomatoes, peppers, and many ornamentals. Tobacco mosaic virus is one of the most common causes of virus diseases of plants in Minnesota.
North America, Europe, Asia, Africa

How to get rid of or kill this disease:
Unlike fungicidal chemicals used to control fungal diseases, to date there are no efficient chemical treatments that protect plant parts from virus infection. Additionally, there are no known chemical treatments used under field conditions that eliminate viral infections from plant tissues once they do occur. Practically speaking, plants infected by viruses remain so. Thus, control of tobacco mosaic virus is primarily focused on reducing and eliminating sources of the virus and limiting the spread by insects. Tobacco mosaic virus is the most persistent plant virus known. It has been known to survive up to 50 years in dried plant parts. Therefore, sanitation is the single most important practice in controlling tobacco mosaic virus.
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Control for Seedling Growers and Gardeners
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The most common method of transferring the virus from plant to plant is on contaminated hands and tools. Workers who transplant seedlings should refrain from smoking during transplanting and wash their hands frequently and thoroughly with soap and water. Tools used in transplanting can be placed in boiling water for 5 minutes and then washed with a strong soap or detergent solution. Dipping tools in household bleach is not effective for virus decontamination. Any seedlings that appear to have mosaic symptoms or are stunted and distorted should be removed and destroyed. After removing diseased plants, never handle healthy plants without washing hands and decontaminating tools used to remove diseased plants.
Persons purchasing small tomato plants for transplanting should beware of any plants showing mottling, dwarfing, or stunting. Avoid the purchase of any affected plant. Gardeners are advised to follow the same procedures recommended for greenhouse workers when handling tomato transplants. Other control methods for home gardeners include roguing (removal of diseased plants), destruction of diseased and infected plants, and control of weeds and chewing insects. When roguing and destroying mature diseased plants from the home garden, be sure to wash hands and decontaminate any tools used in the process before contacting healthy plants.
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Control for Commercial Producers
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Commercial greenhouse producers of tomatoes should follow control practices for seedling production as stated above. It is essential for commercial growers to constantly inspect and rogue diseased production plants while the plants are in the seedling stage. An experienced individual, who is familiar with the tobacco mosaic virus symptoms, should do the initial inspection.
Roguing of young production plants is recommended and should take place before workers are allowed to prune or tie up production plants. When removing diseased plants, also remove one plant on either side of the diseased one. The reason for this is that it is almost impossible to remove a diseased plant and not contaminate the healthy adjacent plants. Never attempt to transplant a healthy tomato into the soil from which a diseased plant was removed. Roots from diseased plants will remain in the soil and provide the virus inoculum for the new transplant.

As a matter of routine, soils from which production plants have been removed, following harvest, should be steam sterilized before the introduction of new seedlings. Steam sterilization can be accomplished by steam or air-steam mixtures. In the preparation of soil for steam sterilization, sift it to remove clumps and large pieces of organic matter. The total soil mixture will have to be heated to a temperature of 200° F for 40 minutes. Since high temperatures are required, steam sterilization must be done in an enclosed system. Temperatures within the steam sterilization system should be monitored by high temperature thermometers to make sure the desired temperature has been reached. Steam sterilization of soil also will eliminate fungi, insects, nematodes, and weeds from the soil. Steam sterilization also is recommended for gravel mixtures used in hydroponic operations following the same procedure described above.
Grow individual production plants in separate containers so that the soil or growing media can be removed when roguing infected production plants. Remember that the soil harbors old root tissues that may serve as inoculum when new roots are introduced. Growing production plants in separate containers is also useful for the control of root diseases caused by fungi and bacteria.
TMV can be treated almost as an organic chemical, rather than an infective agent. "
 

HeartlandHank

Well-Known Member
I have been looking up some stuff on TMV out of curiosity. I have yet to find anyone who tested a cannabis plant and the test was positive.

All of the symptoms I have read and seen pictures about are also symptoms of other conditions, deficiencies, stresses, whatever. Most of which I see in this garden. But, maybe I have the TMV.

Given the strong opinions folks have about it on both sides, I feel like there is much room for an exaggerated risk or lack of risk to circulate. For example, the author of the post up there feels people on the other side of the fence "cannot grasp the idea" and are "nuimbskulls".
 

hexthat

Well-Known Member
have you found any that were even tested or how about any that were ever tested negative??
 

HeartlandHank

Well-Known Member
have you found any that were even tested or how about any that were ever tested negative??
many tested negative.

google: TMV test cannabis forums.

You'll see many many threads of people sighing in relief, negative. I can't find one test positive.

Not saying that I feel it is not a legitimate threat... but I think there has been A LOT of talk about it lately. I wonder just how serious the threat really is.

Also wondering what the consensus is on what to do about it if you do have it. Cut down everything, destroy equipment?
 

HeartlandHank

Well-Known Member
http://www.icmag.com/ic/showthread.php?t=229202
https://www.rollitup.org/marijuana-plant-problems/428775-tobacco-mosaic-virus-tmv.html

http://www.icmag.com/ic/showthread.php?t=242071
https://www.thcfarmer.com/community/threads/tmv-the-final-answer.33713/ (this link gives 3 tests, different growers, all negatives.

There is many many more. Like I said, google: TMV cannabis test positive negative

Find a positive test and post it if you can find it.

People are also saying that there are many different virus, the tests are not accurate, so on and so on. Apparently there are labs that will do more accurate tests for the $. Have seen people say that accurate diagnosis can only be done with an electron microscope.

The problem is, the symptoms everyone describes are not just symptoms of TMV, but other things as well. I can say that most of the symptoms are in this garden and one point or another.

MANY people talk about ChemD being infected.
 

HeartlandHank

Well-Known Member
I've heard about the mosaic virus for a while. Like, don't buy store bought potatoes and use them to start plants, as the industry is infected with the virus. So on and so on.

I'm just wondering how serious the threat is. I guess if you are involved in breeding, and care about the future of cannabis, it should be something you think about. Just like landscapers need to be disenfecting chains saws, hedge trimmer, tree trimmers, etc, when moving from property to property. Many do not take the time and spread diseases around. 409 it, wait 30 seconds, wipe it dry, lubricate. That's all it takes to significantly cut down on the spread of diseases from property to property.
 
many tested negative.

google: TMV test cannabis forums.

You'll see many many threads of people sighing in relief, negative. I can't find one test positive.

Not saying that I feel it is not a legitimate threat... but I think there has been A LOT of talk about it lately. I wonder just how serious the threat really is.

Also wondering what the consensus is on what to do about it if you do have it. Cut down everything, destroy equipment?

Even if you do that. Apparently it can be in the seeds themselves. So you can detroy everything, start over and get a seed that is infected. The entire problem starts all over again. Atleast that is from what I understand.
 

hexthat

Well-Known Member
http://www.icmag.com/ic/showthread.php?t=229202
https://www.rollitup.org/marijuana-plant-problems/428775-tobacco-mosaic-virus-tmv.html

http://www.icmag.com/ic/showthread.php?t=242071
https://www.thcfarmer.com/community/threads/tmv-the-final-answer.33713/ (this link gives 3 tests, different growers, all negatives.

There is many many more. Like I said, google: TMV cannabis test positive negative

Find a positive test and post it if you can find it.

People are also saying that there are many different virus, the tests are not accurate, so on and so on. Apparently there are labs that will do more accurate tests for the $. Have seen people say that accurate diagnosis can only be done with an electron microscope.

The problem is, the symptoms everyone describes are not just symptoms of TMV, but other things as well. I can say that most of the symptoms are in this garden and one point or another.

MANY people talk about ChemD being infected.
All of them links have no evidence.

https://www.thcfarmer.com/community/threads/tmv-the-final-answer.33713/
is obviously not even TMV

You got some issues.
 

hexthat

Well-Known Member
Even if you do that. Apparently it can be in the seeds themselves. So you can detroy everything, start over and get a seed that is infected. The entire problem starts all over again. Atleast that is from what I understand.

Yes this is true. A lot of novice seed banks spread it like "Cali Connection Seeds" and "Reserva Privada Seeds". your better off getting seeds from Nevil's old bank.
 

hexthat

Well-Known Member
Here are some pics of this "Begomovirus" AKA mosaic virus


BEAN
POST PRE

OKRA
POST PRE


LOOKS OK BUT...




PEACH
POST
PEPPER
POST PRE
SQUASH
POST PRE
 

Nightmarecreature

Active Member
TMV infected seeds can be used. Soak seeds in 10% Trisodium Phosphate ( Lowes carries it) for 20 min. The outside hull of the seed carries the virus. The inside of the seed does not. The Trisodium Phosphate will kill the virus. The only way to cure an infected plant is through tissue culturing. Using an extremely small but fast growing shoot will ensure a 90% TMV free plant. Blistering of the leaves on plants is a sure sign of TMV.

Extra Magnesium seems to keep it at bay. I foilar feed at 10% magnesium once a week. Don't go over 10% unless you want the leafs to dry out and get crispy. I have Chem D and according to I believe swerve, it tested positive for TMV at a University.
 

hexthat

Well-Known Member
TMV infected seeds can be used. Soak seeds in 10% Trisodium Phosphate ( Lowes carries it) for 20 min. The outside hull of the seed carries the virus. The inside of the seed does not. The Trisodium Phosphate will kill the virus. The only way to cure an infected plant is through tissue culturing. Using an extremely small but fast growing shoot will ensure a 90% TMV free plant. Blistering of the leaves on plants is a sure sign of TMV.

Extra Magnesium seems to keep it at bay. I foilar feed at 10% magnesium once a week. Don't go over 10% unless you want the leafs to dry out and get crispy. I have Chem D and according to I believe swerve, it tested positive for TMV at a University.
not 100% although most seedling infections are from the shell if the mother was infected all the seeds are infected.
 
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