DNAprotection
Well-Known Member
Ok Frank, or FFF (frank ferret face) for short...for example why have you still not responded to this,Listen, you made retarded claims, that puts the onus of proof on you.
So far you've dicked about and talked shite, but have presented nothing to back up your assertions, infact one of your "sources" completely countered the point you were trying to make.
So...ANY proof at all to back up what you're saying or just more cock-swaggle?
[SIZE=+2]Killing Cannabis with mycoherbicides[/SIZE] [Also see: http://mycoherbicide.net/MUTABILITY/mutability.htm] John M. McPartland, D.O., M.S.
VAM/AMRITA
53 Washington Street Extension
Middlebury, VT 05753, USA David P. West, Ph.D.
GamETec
363 S. Warren Street
Prescott, WI 54021, USA
Abstract [SIZE=-1]Last year, researchers were funded by the U.S. government to create fungi that destroy drug plants, including marijuana (Cannabis species). The fungi will be genetically engineered. Controversies surrounding this "new solution" for the war on drugs are discussed, including the ethics of exterminating plant species that have occupied central roles in human culture for thousands of years. The importation of foreign fungi into new habitats is fraught with unpredictable environmental pitfalls; exotic pathogens can spread from their intended targets to other organisms. All known pathogens of marijuana also attack hemp; exterminating drug plants will probably spell the demise of a valuable and resurgent fiber and oil-seed crop. Genetically transformed fungi are genetically unstable and mutate easily. Fungi with recombinant DNA may reproduce with native fungi and create new strains of virulent, transgenic pathogens. Once these pathogens are released in the environment, they cannot be recalled. In summary, we argue that research involving transgenic pathogens of Cannabis is a dangerous misuse of biotechnology, and call for an immediate moratorium.[/SIZE]
Introduction [SIZE=-1]The U.S. Congress recently appropriated $23 million dollars to fund a "new solution" for the war on drugs. The new solution attacks drugs at their source the drug plants. Researchers say they can eliminate drug plants with fungal pathogens. The fungi would be genetically engineered to kill only coca plants (Erythoxylum species), opium poppies (Papaver species), and marijuana (Cannabis species).[/SIZE] Rep. Bill McCollum, who introduced the appropriation bill, described the tactic as "a silver bullet in the drug war" (Fields 199. The development of transgenic coca and opium pathogens began several years ago, but previous appropriations were relatively small (the 1998 budget was $2.58 million). This year McCollum expanded the program to include marijuana, and moved the budget's decimal point to the right.
A fungal weapon for the war on drugs is not new. Millions of dollars were spent in the 1970s in a world-wide search for fungi which would attack coca (Lentz et al. 1975), poppies (Schmitt & Lipscomb 1975), or marijuana (Ghani et al. 197. See Figure 1. It was a strange era for plant pathologists. While researchers around the globe attacked the pathogens of poppies and hemp, US-funded scientists reversed the strategythey attacked poppies and hemp with the same pathogens (Doctor 1986).
Renewed interest in fungal pathogens for the war on drugs concerns us in its own right. Our law-enforcement lobby wishes to exterminate three plant species that have occupied central roles in human culture for thousands of years. Are the targeted plants inescapably evil? Are there no alternative means for reducing their dangers to humans? We wish to report the ethical and scientific controversies pertinent to this issue, framed for consideration by academia, State and Federal government agencies, and others interested in genetically engineered organisms, biological control, and the drug war (Cook et al. 1996).
Killer fungi [SIZE=-1]Experiments with fungi to control plants began in the late 1960s. The initial targets were noxious agricultural weeds that had been accidentally imported from one region of the world into another, where they became more aggressive, because their natural enemies were often absent. Hence, the classical strategy for biocontrol of weeds involves the importation of natural enemies from their native ranges. Classical biocontrol generally enjoys wide approval and is used by organic agriculture, although the strategy does have its critics (Howarth 1991).[/SIZE]
Classical biocontrol of marijuana was originally envisioned by Arthur McCain in 1970 (Shay 1975). McCain, a professor at the University of California-Berkeley, suggested, "Just introduce a couple of pounds [of a pathogenic fungus] into an area, and while it wouldn't have much of an effect the first year, in several years it would spread throughout the country with devastating results" (Zubrin 1981). In reality, however, classical biocontrol rarely extirpates a weed, it merely reduces the weed population to a low level (Watson 1991). Reduction without eradication is acceptable for most agricultural weeds, but is unacceptable for "zero tolerance" drug control, which seeks the complete eradication of a crop.
The other biocontrol strategy, inundative release, is also called the mycoherbicide approach. This strategy releases massive amounts of fungal spores upon target plants. The mycoherbicide approach can totally eradicate a field of drug plants. This approach, however, utilizes a delivery system similar to that of chemical herbicides such as hovering over clandestine fields in a helicopter while releasing the control agent. Thus the mycoherbicide approach, compared to the current herbicide strategy, is equally expensive, exposes pilots to equal danger as they hover over fields, and may require annual retreatment of annual crops. The mycoherbicide approach is not the suggested "silver bullet."
Fear of foreigners [SIZE=-1]The importation of foreign fungi into new habitats is fraught with controversy. Once a self-perpetuating fungus has been released, it is impossible to recall or control (Lockwood 1993). Despite host-range testing to identify potential nontarget hosts, exotic fungi can spread from their intended targets to other plants. The entire flora of a continent may ultimately be exposed, especially if the fungus produces wind-borne spores (Auld 1991). Because of this concern, only two exotic fungi have ever been intentionally imported into North AmericaPuccinia chondrillina and Puccinia carduorum.[/SIZE]
[SIZE=-1]Fear of "collateral damage" to nontarget plants is justified. When Puccinia xanthii, considered a selective selective pathogen of Xanthium weeds, was imported into Australia from North America, the fungus spread to sunflowers (Helianthus annuus) and Calendula officinalis (Auld 1991). Native fungi sold as mycoherbicides may also spread to new hosts after release. For example, Colletotrichum gloesporioides f. sp. aeschynomene (Collego®), one of only three mycoherbicide fungi commercially available in the U.S., has a wider host range than originally determined, including several economically important legumes (TeBeest 198.[/SIZE]
[SIZE=-1]The situation with fungi is comparable to that with insects. Turner (1985) estimated that 21% of biocontrol insects intentionally introduced into North America have spread to non-target native plants. For instance, the beetle Chrysolina quadrigemina was imported into North America to kill weedy St. John's wort (Hypericum perforatum), but it subsequently moved to the ornamental species Hypericum calycinum (Turner 1985). Howarth (1991) described nearly 100 cases where errant biocontrols have driven non-target hosts to extinction, mostly in island ecosystems. Howarth claimed that more species extinctions have been caused by biocontrols than by pesticides.[/SIZE]
[SIZE=-1]Non-target hosts at greatest risk to exotic biocontrol fungi include:[/SIZE]
[SIZE=-1]1) plants phylogenetically related to the target species,[/SIZE]
[SIZE=-1]2) plants with secondary compounds or morphological features similar to the target species,[/SIZE]
[SIZE=-1]3) plants attacked by fungi related to the biocontrol fungus,[/SIZE]
[SIZE=-1]4) plants never exposed to the biocontrol fungus,[/SIZE]
[SIZE=-1]5) plants whose fungal pathogens are unknown (Watson 1991).[/SIZE]
[SIZE=-1]The study of fungus-host specificity is site-dependent. That is, each potential release site has its own unique flora, fauna, and climactic conditions. Sites with a high degree of biodiversity, such as Amazonia, are teeming with potential non-target hosts. Studies of tropical sites are very complicated and become susceptible to errors of tremendous consequence. The potential spread of fungi away from release sites must also be taken under consideration. Biocontrol agents do not recognize international boundaries, yet host specificity studies rarely consider non-target hosts in neighboring countries (Lockwood 1993).[/SIZE]
[SIZE=-1]In the case of pathogens of Cannabis, the non-target host at greatest risk, because of its close phylogenetic relationship to Cannabis, is hops (Humulus lupulus). At least 10 fungal pathogens are known to mutually infect Cannabis and Humulus (McPartland 1992). The next closest relatives are the Urticaceae (members of the nettle family) and the Moraceae (mulberry family), with which Cannabis shares at least 20 fungal pathogens (McPartland 1992).[/SIZE]
[SIZE=-1]The species debate[/SIZE] [SIZE=-1]The non-target host at greatest risk is Cannabis itself. Within the genus we find plants cultivated for drugs (marijuana), or fiber (hemp), or seed oil (seed hemp), as well as wild, uncultivated plants (feral hemp). How closely related are these plants? Some taxonomists describe marijuana and hemp as completely separate species (Schultes et al. 1974), whereas other taxonomists say they are the same species, Cannabis sativa (Small & Cronquist 1976).[/SIZE]
[SIZE=-1]This "species debate" achieved semantic importance during the 1970s (Small 1979). Drug libertarians promoted the polytypic approach and cited marijuana as Cannabis indica, to argue that statutes written against Cannabis sativa did not apply to marijuana. Conversely, law enforcement agencies have maintained that the genus is monotypic. Now, to rationalize the mycoherbicide approach, law enforcement appears to have reversed its position. Semantics aside, most fungi that attack marijuana also attack hemp (McPartland 1995b, 1995c, 1997, McPartland & Cubeta 1997).[/SIZE]
[SIZE=-1]Clearly, the greatest concern surrounding biological control is host specificity. Consider Pseudoperonospora cannabina, a marijuana pathogen promoted by biocontrol researchers (Zabrin 1981, McCain & Noviello 1985). P. cannabina may be identical to Pseudoperonospora humuli, a pathogen of hemp and hops (Hoerner 1940). We have re-investigated several fungi that were originally described as specific pathogens of Cannabis, but under closer scrutiny, turned out to be misidentifications of widespread pathogens that attack many hosts (for example, "Pleosphaerulina cannabina" turned out to be Leptosphaerulina trifolii, "Stemphylium cannabinum" = Stemphylium botryosum, "Sclerotinia kauffmanniana" = Sclerotinia sclerotiorum) (McPartland 1995d).[/SIZE]
[SIZE=-1]Genetic engineering[/SIZE] [SIZE=-1]Wishing to improve host specificity and toxicity of fungal pathogens, researchers are now turning to genetic engineering (Brooker & Bruckart 1996). The use of transgenic organisms, however, elicits a new set of concerns (Levin & Israeli 1996). These are concerns that resulted in the Asilomar moratorium on genetic engineering of human pathogens.[/SIZE]
[SIZE=-1]Genetic engineers have recently been investigating a coca pathogen, Fusarium oxysporum f. sp. erythroxli (Sands et al. 1997, Nelson et al. 1997). F. oxysporum f. sp. erythroxli was selected for coca eradication because it caused natural epidemics in Peru and on the former Coca-cola plantation on Kauai, where "containment of the fungus proved challenging" (Sands et al. 1997). Fusarium oxysporum is well known to bioengineers, and previous researchers successfully inserted toxin genes into the species (Kistler 1991). Nevertheless, Gabriel (1991) considered it "unwise" to clone a toxin gene into a necrotrophic pathogen (such as F. oxysporum). He argued that such a pathogen might gain unexpected fitness and radically expand its host range, "a potentially dangerous experiment." Fusarium species can produce a variety of toxic metabolites known as trichothecenes, which gained some notoriety for their reputed use in biological warfare ("yellow rain"). F. oxysporum is known to cause systemic infections in humans (Rippon 198.[/SIZE]
[SIZE=-1]Genetically transformed fungi have unstable genotypes, making mutations more likely. Experiments have shown F. oxysporum spontaneously mutates its transgenic DNA (Kistler 1991). Furthermore, F. oxysporum utilizes parasexual coupling, and at least 5% of its genome consists of transposons, or moveable pieces of DNA (Kistler 1997). Parasexuality and active transposable elements would facilitate the transfer of recombinant DNA to native fungi, potentially creating new strains of virulent pathogens. The wheat pathogen Puccinia graminis, for instance, hybridizes with other fungi on wild grasses, giving rise to offspring with increased virulence (Luig & Watson 1972, Burdon et al. 1981). This fact is not cited by proponents of biocontrol with rust fungi (Cook et al. 1996).[/SIZE]
[SIZE=-1]"Gene flow" has been more thoroughly studied in plants than fungi. Levin & Israeli (1996) documented five examples of spontaneous gene flow from crops to native plants, which resulted in new or worse weeds. The introgression of engineered genes from transgenic crops to related weed species has been demonstrated (Brown & Brown 1996), and may arise after just 2 generations of hybridization and backcrossing (Mikkelsen et al. 1996).[/SIZE]
[SIZE=-1]Currently, testing for gene flow is not standard procedure during the evaluation of transgenic organisms. This could be accomplished by crossing engineered fungi with related fungi (particularly if the fungi reproduce sexually, and especially if they are heterothallic fungi). Several generations of crossed hybrids are evaluated in serial host studies. Testing for gene flow is especially imperative for biocontrols which have been genetically manipulated to resist fungicides. Researchers have transformed Colletotrichum gloesporioides f. sp. aeschynomene (Collego®) with a gene for fungicide resistance (Brooker & Bruckart 1996). Imagine if this fungicide-resistant gene introgressed into Histoplasma capsulatum or other human pathogens commonly found in agricultural areas![/SIZE]
[SIZE=-1]The species question, round two[/SIZE] [SIZE=-1]Another Fusarium species, F. oxysporum f. sp. cannabis, is the primary candidate to kill marijuana (Hildebrand & McCain 1978, Noviello et al. 1990) and wild hemp in the American Midwest (Shay 1975). See Figure 2. Researchers promote F. oxysporum as a marijuana mycoherbicide because they claim hops, Humulus lupulus, is not susceptible to fusarium wilt (McCain & Noviello 1985). They overlooked "Hops wilt" caused by F. oxysporum in Australia (Sampson & Walker 1982).[/SIZE]
[SIZE=-1]F. oxysporum [/SIZE][SIZE=-1]f. sp. cannabis was originally isolated from hemp cultivars in Italy, by researchers who believed "...the wilt disease and its pathogen have not been previously described" (Noviello & Snyder 1962). In fact, these researchers missed many previous descriptions of this wilt disease (Dobrozrakova et al., 1956, Rataj 1957, Ceapoiu 1958, Czyzewska & Zarzycka 1961, Barloy & Pelhate 1962, Serzane 1962). All previous descriptions attributed hemp wilt disease to Fusarium oxysporum f. sp. vasinfectum. This fungus is morphologically identical to F. oxysporum f. sp. cannabis but has a very broad host range (e.g., cotton, mung beans, pigeon peas, rubber trees, alfalfa, soybeans, coffee, tobacco and many other plants).[/SIZE]
[SIZE=-1]McPartland (1995a) proposed that F. oxysporum f. sp. cannabis may be a misidentified pathotype of F. oxysporum f. sp. vasinfectum. Similarly, the fungus causing tobacco wilt, originally named F. oxysporum f. sp. nicotianae, proved to be a race of F. oxysporum f. sp. vasinfectum (Armstrong & Armstrong 1975). According to Kistler et al. (199, F. oxysporum f. sp. vasinfectum consists of at least 10 vegetative compatibility groups (VCGs). Comparing F. oxysporum f. sp. cannabis with the genotype of F. oxysporum f. sp. vasinfectum can be accomplished with VCG studies using nit mutants.[/SIZE]
[SIZE=-1]Conflicting interests[/SIZE] [SIZE=-1]U.S. regulations have prevented the testing of bioengineered fungi in the field (Brooker & Bruckart 1996). But regulatory oversight is lacking in Peru and Columbia (Levin & Israeli 1996). Exigencies generated by the drug war metaphor could dangerously rush these fungi into deployment.[/SIZE]
[SIZE=-1]Moreover, saboteurs or irresponsible scientists could breech regulatory barriers, as occurred in Montana where several bioengineered organisms were illegally released around 1987 (Roberts 1987). In Australia, saboteurs illegally introduced the fungus Phragmidium violaceum to control European blackberry (Rubus fruticosus). Weedy R. fruticosus was spreading across pastures and impeding Australian cattle ranchers. The government had previously rejected ranchers' requests to import P. violaceum, because of economic objections from commercial blackberry growers and beekeepers. Wind-borne spores of illegally introduced P. violaceum dispersed rapidly across the continent, and the fungus now infests at least four Rubus species (Watson 1991).[/SIZE]
[SIZE=-1]The Australian debacle illustrates how biocontrol may impact competing interests. The first U.S. drug czar, Carlton Turner, recognized that target plants may be considered noxious weeds by one group, and valuable crops by another group (Turner 1985). St. John's wort (Hypericum perforatum) is an excellent example. H. perforatum was previously branded a noxious weed. But now it has become the second-best-selling herbal medicine in the U.S. $121 million dollars of H. perforatum was sold last year, and producers are predicting a severe shortage of raw material (Brevoort 199.[/SIZE]
[SIZE=-1]As consultants to the European and Canadian hemp industry, we face a dilemma. As ecologists, we endorse classical (non-engineered) biocontrol organisms as potential replacements of chemical pesticides (McPartland 1984, Doctor 1986). As physicians, we praise the safety of biocontrols over paraquat and other synthetic herbicides (McPartland & Pruitt 1997). Nearly 20 years ago, these reasons guided our decision to search for classical biocontrols against marijuana (McPartland 1983). But times have changed. Hemp cultivation has resurged in western Europe, the former USSR, and China. Last year the Canadian government allowed farmers to grow hemp for the first time in 50 years 251 farmers successfully harvested 5,930 acres (Cauchon 199. Have our neighbors to the north been explicitly informed of the "Western Hemisphere Drug Elimination Act" spearheaded by Rep. McCollum? We feel the development of transgenic mycoherbicides against marijuana would endanger hemp cultivation, permanently. Hemp is usually a pest- and disease-tolerant crop requiring little or no pesticide for cultivation; it has been characterized as "an environmentally friendly crop for a sustainable future" (Ranalli1999). Hemp should not be endangered, and research involving transgenic pathogens of Cannabis should be halted. Moreover, the use of genetically engineered pathogens as a weapon in the drug war should be reevaluated.[/SIZE]
[SIZE=-1]Acknowledgements[/SIZE] [SIZE=-1]We thank David Morris and two anonymous phytopathologists for reviewing and improving our manuscript.[/SIZE]
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[SIZE=-1]Figure 1.[/SIZE]
[SIZE=-1]Healthy marijuana seedling flanked by plants exposed to pathogenic fungi.[/SIZE]
[SIZE=-1]
[SIZE=-1]Figure 2.[/SIZE]
[SIZE=-1]Microscopic spores of Fusarium oxysporum, a potential mycoherbicide of Cannabis.[/SIZE]
[SIZE=-1]