found this info for your quest :
[FONT=Arial, Helvetica, sans-serif][FONT=arial,helvetica,sans-serif] Biochem 101: How to design a Cannabis-equivalent citrus plant [/FONT] [FONT=arial,helvetica,sans-serif]Step One:
Biochemically isolate all the required enzymes for the production of THC.[/FONT]
[FONT=arial,helvetica,sans-serif]Step Two:
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes. [/FONT]
[FONT=arial,helvetica,sans-serif]Step Three:
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium. [/FONT]
[FONT=arial,helvetica,sans-serif]Step Four:
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
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[FONT=arial,helvetica,sans-serif]hope it helps
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