wzntez

when i started i used crushed vitamins and store gelatin, fert sticks ! actually some success too. the real trick is using less, not more, until you see the end results. my expectations are different than yours, I grow trees, you may want a sog, etc. the hardest part, is sterilizing the plant material, enough to kill all bacteria, without destroying the dna. water. make sure the explant has been stripped of its leaves and outer layer then 70% alcohol for a couple minutes, rinsed for 5 minutes in sterile water then 5%bleach/water for 30 seconds, rinsed for 15 minutes in sterile water, then transferred

you're welcome. I have posted most of what I do, except the specific mix ratios of nutrients/hormones/agar/sugar to water. If you start with a meristem growing tip midway plant and the recipe for carrot callus, you'll get acceptable beginner results with callus culture, from there it becomes an understanding of the four hormones, their purpose, and your intention. one is the clone dip we already use, this will induce root formation if mixed into the agar with the other necessaries. hint--its probably already diluted 200:1 and one half of one ml woud be enough to see results in a litre of mix.