Cloning Plants By Tissue Culture

YEAH i know ITS CAUSE of the mg/l-10 (1.0mg/l-10) I KNOW!! ITS fun though right!! tried to figure it out without bothering the thread to much with math....but it came to be that hard wood & mj were requiring 1.0 to 2.5 ppm iba...
SO thats what we went with this time or close..tiny drops,,, defiantly 20 drops per ml,,,3-4 drops..im good...witch reminds me,,how are they doing..done at 100am...

i do use cyto and auxi at the same time, most of the time, unless something is needing tweaking

this time i treated with dip n grow 12 hrs.....my 1st. medium batch i put in 15 drops dip n grow and there all growin some-what..so to much will be the determining growing factor here or to little... eye lashes,,,not a problem..my transfer area is NEWBIE SAFE!! I HOPE..

pharmacoping said:

i do use cyto and auxi at the same time, most of the time, unless something is needing tweaking

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Do you use TDZ? and you recommend around 200ppm complete nutrient? Whats your position on the B vitamins?

Guile,
My experiences is about the same with established plants.. I haven't killed one yet but it can sure mess up yields when done wrong... ,
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Unless outside, my results were never ever consistant, until I built the right room/combined with proper genetics , with the right controls, since then(3yrs) I have had no surprises, no issues, no variances, no failures or deaths or late flowers, and almost total automation.. its totally about the environment we provide, once stable and constant and correct, they flourish every time. I have no regrets on spending the money to do it once/right, after decades of home depot growing with varying results

i have no experience with tdz. I use 150ppm when growing or storing callus material, 200ppm for rooting/shooting. never higher than 200ppm for me in vitro.yrs ago I used crushed vitamins, so sure B was there. But in recent yrs I use Eco salts and they contain every needed major and minor vit/min necessary for plant perfection, however, will affect ph, which should be adjusted before sterilizing. I have never personally added thrive alive to anything on a regular basis.I used it once in a free trial kit from technoflora, but that was it. I have no position on them in tissue culture. But like in any hydro application, if your nutrient is lacking, and additives are needed,why not use a nutrient that is complete?

pharmacoping said:

i have no experience with tdz. I use 150ppm when growing or storing callus material, 200ppm for rooting/shooting. never higher than 200ppm for me in vitro.yrs ago I used crushed vitamins, so sure B was there. But in recent yrs I use Eco salts and they contain every needed major and minor vit/min necessary for plant perfection, however, will affect ph, which should be adjusted before sterilizing. I have never personally added thrive alive to anything on a regular basis.I used it once in a free trial kit from technoflora, but that was it. I have no position on them in tissue culture. But like in any hydro application, if your nutrient is lacking, and additives are needed,why not use a nutrient that is complete?

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Just picking your mind... Its nice to be able to hold a conversation with and informational resource..
hat Cytokinins do you use? What concentrations? What are Eco salts?

bap,naa,kinetin,iba,ppm,1 mg/ml concentration somewhere in this thread I listed the four hormones, and their concentration I purchase them dilute. I cant post "my personal" recipes, but will say that the china man is spot on for most application, albeit a little slow. I dont know how much is in the diute mix, I use drops per litre when mixing, with pipettes showing measurements. until the last transfer before going to veg, there are no hormones or sugar, just agar and nutrient and ppmgoogle ECO hydroponics and you'll find his website. he sells nutrients in pure basal salt formulations.

K so there all showing signs of growth (1st batch) 3 of them are bout the same...I dont know,,, but,,,, i think i have a GOOD SIGN..The ones that i did last night seem to NOT have the liquid ("dont do it grasshopper") that the last ones have...i think that i see the signs of the break down of the agar,,,but this is about the time that the NADCC totally wears off,,so transfer is soon....this is my 2 week transfer to new medium with the aux/ coyt. or a small amout of eco salts+the same iba and such from the first medium.>>>>I THINK....
I know about ppm...and i plan on getting it....my specimens are mostly clean other then some normal white mold...AGAIN I THINK.....
I WILL HAVE RESULTS WITH THIS, THE WILL TO SUCCEED IS OUT MATCHED BY ODDS OF FAILURE

Or seriously we need to get some internet cops so that some people wont have to wade threw all the BS to find one honest sight that gives it to you like it is...sellin thangs' is not making the economy better,,,it may seem that way, but man oh man,,, at the end of the year when u make that dump run,,, it sure is hard throwin all that money away HUH??? the thing is,, id be willin to spend ALOT of money at a place that would NOT try to sell me that ONE product that is SHIT,,and they know it....or the same as the other guy but with a cooler label... u know what i mean RIGHT..i have a high shopping/money spending moral code.....ONCE and its on the company and NEVER will i give them MY money EVER AGAIN....BUT THATS NORE HERE OR THERE...

No sugar????? When u transfer to new medium do u wash them again..i guess u would right..

white mold aint normal, should be no cloudy,fuzzy, color bleeding, red ooze, or discoloration of any kind on or near the tissue sample. it should look like clear agar, and an explant in it. If not, discard, start again. young ones wil not survive fungi. plus fungi is now in your sterile culture environment. remove it quickly. older cultures have a better chance. you'll need to clean your explants better. almost there dude !!

NO NO NO not in the jars NO NO!!!! just a leaf or two on the original potted mother... NO NO were good there..I washed the shit out of them in the nadcc and in diluted alcohol...i was very clean and and titty... if i would have had any signs like that HOLY SHIT......NO NOT in the jars.

AWESOME ! good to hear, I was scared for a minute. so you got the powdery mildew... i got a brand new sulphur vaporizer in my rooms with a nite time only plug on my room controller that I've never used, and sulphur pellets, for the just in case...whew. they do make a simple spray to cure it though i never used it either. good to get rid of asap, before it infiltrates flowers, and slow veg growth

peace

That mold was on my friends plants not mine,,,,but thats WHAT DID HAPPEN!!got some stuff for it cause of the clones and bam,,dead, not the mold either,, my pore clones,, most of them..Thats what i mean,,,SELL me anything cause they got to pay the bills,,,im not payin bills,, im play around the bills,,theres a huge difference,,,If if did this for cash,,,,or to pay the bills the guy would have fukd me...thing is hes the only game here so if i needed something fast say a bulb,,well then i cant cross his store out and take my business else where..oh well..

gonna make a new batch now but ive done some research on this additive i have for my rooms and WELL...aux. cyot.. YYYYEEEEEYYYY.......im startin to think about all the things i read from ur posts and im getten it...couldnt say it all at once though... almost ur hole recipe ,,except for,, how much of,, is all right in this thread... mabye 1 special thing u might add thats ur thing,,but its here,,some where..huh.... sssshhhhh... dont tell people how fukin cool this is..next is ppm and more lighting/storage,,cause those thangs are growin,,perky tips and weird growth happening, little leaves..mind you that i cut ALL the greenery off most and now i see little growing thangs and bigs ones to.....one of them,, i do see some spots ,,, they are in the medium kinda in a group of 4to 6 but the culture is one of the healthiest.. its time to trasfer to new medium,,,the ppm wont be here in time so the nadcc will give me 4-7 days longer..that was the CHEAP part about that nadcc was 4-7 days....then im back to spendin money making mediums more often $$$$(that was alot of Ms).
so i guess i would wash the cultures off AGAIN when i re-jar them? in a solution or sterile water?

hell yah,,,thats me,,,not about TC but a custom built from the GNRs' creations work bench..60+mph 30%+ nitro rigger...soon to come 1/10 scale v-drive drag boats,,, 137ft. track 75+mph..


the cultures got growth out of the tops the cut ends..this i did not see this morning,,their little leaves...but i definitely see black spots appearing in the medium by but not touching the stem,,
i know its time to get them in the NEW medium batch,,,added some stuff,,less sugar and 2 drops of?????with the dip n grow..well see!! A MUST HAVE FOR THIS IS PLANT PRESERVE,,,nadcc will have me makin medium ever 4-5 day for the sane TCs,,SAME money spent,,,,bottom line all you TC grasshoppers plant preserve is a must!!!!
and for all you who just tuned in im doing this without a kit,,,BUT if this works then think about a dude that KNOWS HIS SHIT-- WITH PICTURE RESULTS who offered a deal like that....mmmmmm

shit that guy might be around HERE!!

there they are!!
test run
pretty good hepa filter 2stage with my fiter at top..

not bad for the first time grasshopper,,,not much but they were stems 8 days ago!!!got a new mix and their all alive and going in there tomorrow..I PUT THE METAL LID UNDER THE PLASTIC THEN I PUT THEM IN A ZIP LOCK AND THEN TAPED THE ZIP PART,,THATS THE WAY GRASSHOPPER!!!

this ones for u gulie or who ever

a note on cleaning the explants: we did rose micropropagation 2 terms in a row, keeping the explants in a 20% bleach solution for each. the ONLY difference was that the irst time, we left our explants in it or 10 minutes, and the second, we did 20 minutes. about 50% of the irst ones got contaminated. NONE! 0%! of the second ones showed any signs o contamination. cool, huh?

note* we did it in an enclosed HEPA hood... not the gloves one, but a hood, still.