Greenpoint seeds!!

Michael Huntherz

Michael Huntherz

Well-Known Member
jayblaze710 said:
Long, slightly off topic, slightly ranty post incoming.

I have very real problems with Phylos. Back in a different life, I did evolutionary genomics research. It involved sequencing random snippets of DNA across the entire genome, and comparing these snippets of DNA from hundreds of individuals against each other to determine their phylogenetic relationships, identifying regions of the genome underlying specific traits, and to identify genomic regions under selection in different habitats. I don’t do anything even remotely similar anymore, but I do have a very strong background in genomics research.

There are some giant pitfalls that can make genomic analysis extremely misleading. This is why scientific papers are extremely long and extremely dry. The majority of the paper is simply outlining the methods used so other scientists can determine if the results are justified or not.

These issues include but are not limited to 3 big things. What region or regions of the genome are being sequenced and analyzed? What algorithms are being used for the analysis? Finally, what is the complete data set used for the analysis?

The data set...without a sufficiently large data set, results can be deceiving. This is why your ancestry.com or 23&me results can change over time. Your dna isn’t changing, their data set is becoming larger and more complete. (Side note - I think those sites are a ripoff).

Algorithms...this is a hugely complex topic, but suffice to say, there are multiple different methods for analyzing genomic data, some are better suited than others for specific analyses or regions of the genome. Selecting the correct algorithm is a very important aspect of any genomic analysis.

What genomic region or regions are being sequenced. It’s important to understand that every organism’s genome doesn’t have one specific lineage. Rather, the genome is composed of thousands and thousands of genomic regions that all have their own distinct lineage. A gene that has undergone strong selection in multiple unrelated populations will lead someone to erroneously deduce that the populations are closely related. For a relationship analysis of closely related plants, you ideally want to sequence from thousands of genomic regions throughout the entire genome. At this point, I have no idea what genomic regions are being sequenced by phylos, but I do have some guesses. In short, I don’t think their analyses are particularly accurate because I don’t think they’re sequencing enough of the genome.

Finally, on to OGs and potential S1’s. For a poly hybrid like the OG (I’m assuming) you should absolutely be able to tell an S1 from the parent with a thorough genomic analysis. You will still get recombination of genes that are heterozygous in the parent, and a good genomic analysis can identify this. This is why I don’t find Phylos’ analyses particularly compelling. If they’re finding all OGs to be identical, then they’re not genotyping individuals to a thorough enough level. They’re likely just sequencing a few genic regions which is an outdated method of comparing relationships of related plants.

Personally, I believe all the pure OGs are either 1) renamed cuts, 2) S1’s of the original Ghost OG from Orgnkid, or 3) S1’s of S1’s. Also, I personally believe the original OG came from a Triangle Kush S1.

Here’s an interesting post from ThaDocta on OG cuts.
https://www.icmag.com/ic/showpost.php?p=7664527&postcount=1849
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I have a layperson’s background in genetics, from some hobbies I have pursued since I was a kid...and I gotta say, legit post @jayblaze710 - thick with uncommon clarity, thank you.
 
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Bodyne

Bodyne

Well-Known Member
Chunky Stool said:
Speaking of ORGNKID's Banana OG, it's looking like Texas Butter could be a killer strain if it finishes well.
It's rated as 'short-medium' but should be 'medium-tall'.
I've got one in a 10 gal that's taller than me (6'1"). :shock:
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Im finding everything I grow from GPS stretches like crazy. For the life of me, I can't figure Chem4 being indica dom, Tres Dawg being indica dom, and the stardog is 90% sat. Im guessin they grew a bunch out, found the most sat one and bred with it. Cause there ain't no way, when I've grown the chem d cut in Salem OR, and the Chem 4 cut in Medford, and in 5 gallon smarties didn't stretch like the GPS stains in one gallon smarties. I am trying the dosidos in 2 gallon smarties. If they keep stretching like they are, I be goin to fem seeds or making my own, and just try to save pollen from the males. Hibernate supposed to be a shorter plant, Texas Butter. Bodega Bubblegum. Skydweller. Ghost Town. I guess I'll try to buy some of these, I bought all the others. Also, I notice today, that Blizzard Bush is listed as LVTK x Stardawg, when did Pure Vida, aka HPK/ Appalachia becpme LVTK? Fuckin moon comin up must gonna be a doozy! LOLZ
 
Blake_n_Concentrates

Blake_n_Concentrates

Well-Known Member
^^ this is why I'm sure the cowboy cookies is going to have an insane stretch and flower time that will probably rival even GMO. lol Almost every early flower pic of greenpoint strains shows crazy stretch, but they also seem to yeild very well in exchange.
 
Bodyne

Bodyne

Well-Known Member
Lurpin said:
I feel your pain... I've been trying to string together a shipment of real cuts out here to the Pacific and the logistics of trying to find someone to ship clones is tiring.

Edit: How does someone like orgnkid get all the cuts and stuff that he was using for his library? Or how does Gu~ get his cuts. It seems like the community is pretty walled off if you ask me. I see people like oni seeds and symbiotic genetics gifting cuts to certain people on IG. It makes me scratch my head like how do I get some..
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Story I heard in Oregon is he had very good folks, maybe related in Oregon to source from that he didn't do hisself, maybe his ole man, not sure, and had great connections in Cali. Cept for Elite Genetics, lol. That might have been a slight mistake, but now I see people crowing about Elites, Chems, suge's pk, etc. Gotta love the canna world.
 
Bodyne

Bodyne

Well-Known Member
Blake_n_Concentrates said:
^^ this is why I'm sure the cowboy cookies is going to have an insane stretch and flower time that will probably rival even GMO. lol
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already bean chuckers selling beans with that GMO as the mom I see elsewhere, just like using the NightRider as a male. I still want from the unknown Great Gatsby bean group that devil's reject. Meat breath and hell breath would make a good male, imho.
 
MrMayhem1134

MrMayhem1134

Well-Known Member
Man has it been a long time since I was here last!! I was asking about seeds and researching them now here I am about a year later from first joining this forum and I am ready to contribute to it! I popped 1 each of Lucky 7's and Sundance Kid. Also popped 2 Sky Dweller a couple months back and got 1 female and 1 male. The female is 6 weeks 5 days in and has some burning, but nothing too bad imo. Follow me on IG for weekly updates and grow journals. IG - NextLevelGarden.

Now a pic of the seeds:
20180718_152758.jpg

I will get a pic of the Sky Dweller tonight!
 
Heisengrow

Heisengrow

Well-Known Member
Bodyne said:
already bean chuckers selling beans with that GMO as the mom I see elsewhere, just like using the NightRider as a male. I still want from the unknown Great Gatsby bean group that devil's reject. Meat breath and hell breath would make a good male, imho.
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Could have gmo 20 times over.it just doesnt impress me of all the pictures I've seen.it might be potent but as far as looks its gonna have to be a no for me.
 
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