stonedmetalhead1
Well-Known Member
I understand all of that so on average how many tests are you running on each submitted sample of bud when you give someone results?
Thanks for coming in and clearing these things up.ALSO A QUESTION TO THE SCIENTIST (if any are out there) = "WHICH STRAIN HAS THE HIGHEST THCV%?!?!?"
I probably should have started another thread with these questions, but this one seems to still be pretty active. Also, most people would word the question for the answer I'm looking for like hazey grapes did.
Hopefully this can stay an spirited/intelligent/positive discussion amongst a people of a united cause, and we leave the spiteful/ignorant/negative debating to the politicians impossible to unite egoistic agendas
the strain is called CBD28 because it has higher levels of cbd...5% cbd...ya ok...
Thank you again. rize up!From the chromatogram of the sample extract alone, you're right, it is not possible to calculate the percentage by weight. However when combining this information with the chromatograms of the standards and the weight of the sample from which the extract is made, these calculations can be made according to the formula I previously posted.
Can you make your own THCV standard using preparative HPLC?I'm familiar with the SC Labs series of videos. They've partnered up with WeedMaps so they've got access to more resources than we do but we plan on producing similar ones targeted towards patients in Michigan.
Unfortunately, there are no commerical standards available for THCV and many of the other minor cannabinoids, however we have made requests with our suppliers to provide these compounds for us. The only other option is to custom purify these compounds in house however this would require the use of a mass spectrometer, LC, and fraction collector. We plan on doing this in the future but lack the mass spec at the moment.
Getting back to your original question, I assume that you're interested in the variability of biological replicates. ...
We've used standards from both Lipomed & Restek. Restek seems to be the way to go for now. Yes, it is possible to make your own THCV standard using preparative chromatography, but first you've got to find plant material rich in this compound. Also, you'd want a mass spec to be able to confirm the compound you have isolated is the one you intended and to rule out the presence of any background contaminants.Can you make your own THCV standard using preparative HPLC?
You using Cerilliant standards?
VVVVV Don't assume. What he is doing is called trolling. He is only looking to prove his preconceived ideas about cannabinoid analysis by peppering you with repetitive/short/leading questions. Notice, you clearly answered his question already, and he asked it again slightly rephrased, classic troll move. He is trying to wear you out, he is a long distance poster, not much substance, but you can't get the last word. Don't let him get to you. Thanks again for dropping the much needed knowledge, especially that simple formula for calculating %. I always have my best buds tested and my #s come up on the high end.
For sure. Steep Hill Lab, Im fairly certain, has done this already using the high THCV Jack the Ripper phenotype.We've used standards from both Lipomed & Restek. Restek seems to be the way to go for now. Yes, it is possible to make your own THCV standard using preparative chromatography, but first you've got to find plant material rich in this compound. Also, you'd want a mass spec to be able to confirm the compound you have isolated is the one you intended and to rule out the presence of any background contaminants.
Its all about the terps. The more I know about cannabinoids and cannabis, the more I know that its not the cannabinoids at all that we care about. Its the terpenoids, flavonoids, esters ect that make up the actual affects. The favorite OGs test way low, Larry OG typically around 12%. Its all about the alpha-pinene in lemony strains, which acts to increase the affects of THC. The difference between agonist terpenoids, and antagonist terpenoids, are what we really care about. But cannabis science is not advanced enough at this stage to be able to look at cannabis from this perspective. So we focus on the big three cannabinoids because they are more straight forward.20-25% thc strain grown correctly will make you one happy camper. Remember only in perfect conditions will it be that high.
Any results posted by someone who is selling seeds can not be trusted.Reading this thread shows me how inconsistently screwed up the whole THC % issue is. Because there's no real standard of measure and other reports by the experts claiming how easy it is get differing results confirms how meaningless the figure is. The problem is only exasperated by seedbanks and growers who want to flaunt their superior self image or boost profits. The only accurate method is personal side-by-side smoking comparisons. To hold your own THC Cup showdown of strains.
I thought we were doing a strain review? Check it out y'all, I don't know about the rest of you but I come to this site to research marijuana. Why, because I believe that many people on this site have first hand knowledge of growing marijuana and have EXPERIENCE that I can learn from.
What I don;t do is come to this site to trade political insight, or to discuss military affairs with those people who do not have first hand knowledge of the subject. Why? Because most people are talking out their ass and have no clue wtf they're speaking on.
I think we need to find some weed that will help a mutherFuker remember wtf they're talking about.