My first class with the golden teacher

polyarcturus

polyarcturus

Well-Known Member
aCiDjEsUs said:
Thank you Poly, I'll get rite on it I have some extra "red" bulbs around. Poly you know that "black" light isn't a really a black light like you got at a club, these are black night lights for reptiles, they emit very little or any usable light just heat but you probably knew that, same with the "red" light same thing, but Thank you and I'll get on it.
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night_heat_lamp_rating.jpg

this is the spectrum for the blue light(exoterra im assuming i have few red and blue laying around, they tend to burn out rater quickly.)
which is a complete lie, because they obviously have a dark colored filter that changes the spectrum. on top of that 630 nm where this light is the highest is the same peak as an HPS, why is it not the same color?

the reds chart looks more correct, to what is seen in the visible spectrum.
http://www.exo-terra.com/en/products/infrared_basking_spot_lighting.php

an even better expample would be to look at the different shades of led from 630nm to 740.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
aCiDjEsUs said:
Matt I'm not giving up yet, I'm not that kind that gives up easy :)
I'll keep them jars for another week maybe two? and will see what happens.

I'm not bashing spores101, just can tell the difference between spores101 and sporeworks.
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Acid, you could almost make 2-3 syringes with that one if it is so heavily populated with spores. you have to remember that you only need one spore to get your grow on, the more the better obviously but there is such a thing as overkill too. personally if i was you i would head out to the local pharmacy and pick up a few more 10cc syringes, make yoursef a jar with some sort of hole in it and high heat silicone to cover the hole. fill the jar with distilled water and pressure cook it for an hour or so. then inject your syringe into the jar once it cools, shake it up and fill up 3-4 syringes.

thats what iwould do if i got a syringe that was so heavily populated, it will make it easier to distrubute them into the jars with minimal waste.

my 2¢
 
canndo

canndo

Well-Known Member
RetiredMatthebrute said:
Acid, you could almost make 2-3 syringes with that one if it is so heavily populated with spores. you have to remember that you only need one spore to get your grow on, the more the better obviously but there is such a thing as overkill too. personally if i was you i would head out to the local pharmacy and pick up a few more 10cc syringes, make yoursef a jar with some sort of hole in it and high heat silicone to cover the hole. fill the jar with distilled water and pressure cook it for an hour or so. then inject your syringe into the jar once it cools, shake it up and fill up 3-4 syringes.

thats what iwould do if i got a syringe that was so heavily populated, it will make it easier to distrubute them into the jars with minimal waste.

my 2¢
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this is incorrect information - one spore will never fruit - ever. Mushrooms have sexes just like other plants and animals, you need two spores that are genetic complements in order to get your mushrooms to grow fruit.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
my apologies, i didnt know that. ty for the correct info.

whats your opinion on making more syringes? i am still a newb with all this so i openly admit i dont know shit
 
canndo

canndo

Well-Known Member
RetiredMatthebrute said:
my apologies, i didnt know that. ty for the correct info.

whats your opinion on making more syringes? i am still a newb with all this so i openly admit i dont know shit
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You should never need more than one clean syringe of spores for each sort of mushroom you grow. You are best getting to monoculture anyway.
 
Thundercat

Thundercat

Well-Known Member
I found making my own syringes to be very easy. Thats how I started my grow, a friend sent me some spore prints he had taken off his grow, and I made up syringes with them. I never got into liquid cultures, or myc transfers while I was growing them. I made syringes a couple times, and each syringe would shoot up a whole batch of jars. As I know I've said in this thread before I like my syringes to be dark so that a few ccs will have plenty of spores in it. I know the idea that spores are microscopic means a clear syringes could still have spores, I know a dark syringe has them! I think I would usually use about half a spore print per syringe, which was prolly overkill but didn't matter I had plenty.
 
canndo

canndo

Well-Known Member
Thundercat said:
I found making my own syringes to be very easy. Thats how I started my grow, a friend sent me some spore prints he had taken off his grow, and I made up syringes with them. I never got into liquid cultures, or myc transfers while I was growing them. I made syringes a couple times, and each syringe would shoot up a whole batch of jars. As I know I've said in this thread before I like my syringes to be dark so that a few ccs will have plenty of spores in it. I know the idea that spores are microscopic means a clear syringes could still have spores, I know a dark syringe has them! I think I would usually use about half a spore print per syringe, which was prolly overkill but didn't matter I had plenty.
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That is all fine, however, you should look into monoculture, this is the very best way to grow as there won't be those internal divisions within the substrate and you can be assured of the same species at every flush. Monoculture is easy to do, especialy if you are making your own syringes anyway. Seems that the hyphae have a fairly long period of viability when imersed in sterile water, there is a way to put small, sterile pieces of glass in a syringe and put a bit of the inner tissue into your syringe, then shake it - a lot. You now have a syringe of a single culture that will quickly grow on any substrate.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
well i just like the idea of being able to store multiple syringes incase something happens and you have to sterilize and start from scratch, instead of ordering new ones you already have them sitting in the fridge!!
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
canndo said:
That is what prints are for.
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yeah but how to you keep prints sterile? i like the idea of syringes because if you take the precautions to have nice sterile syringes they will store forever and you dont have to worry about contams once they are capped. with a print isnt there the chance that "other" spres will land on the print paper and get acess to your sterile enviorment?

again im still learning so...
 
polyarcturus

polyarcturus

Well-Known Member
RetiredMatthebrute said:
yeah but how to you keep prints sterile? i like the idea of syringes because if you take the precautions to have nice sterile syringes they will store forever and you dont have to worry about contams once they are capped. with a print isnt there the chance that "other" spres will land on the print paper and get acess to your sterile enviorment?

again im still learning so...
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wrap them in foil label them and put them in a baggie or container. prints are better than a syringe it will last longer.
 
Thundercat

Thundercat

Well-Known Member
If you take your prints right, and keep them closed up then they should stay sterile. Obviously when you open them there is always a chance of messing it up, but I suppose it comes with the territory. The idea of the monoculture sounds great, your basically cloning the mushroom if I understand correctly. Again I just never got that far, my project lasted about 4-5 months total. Then between a series of unfortunate events it was time to shut down. I look forward to the day I may be able to learn to isolate strains, and play with them all again.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
Poly, what im saying is that a syringe is more apt to stay sterile than a print just wrapped in foil...

the syringe is completly enclosed and air tight...the foil has more air leaks and cracks for contams to get into...

glove box and sterile distiled water = sterile sringe?

i dunno, maybe im just taking the sterile to too much of an extreme.....
 
polyarcturus

polyarcturus

Well-Known Member
bringing the water to the scene is where contmination occurs, foil is pretty good at sealing if you fold the edges. but really while your in the sterile environment you should be taking that foil and placing it into sterile bag. to completely seal it.
 
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