"I keep seeing things that look more like what I would call "micro-propagation" than cell culture.. Maybe My impression of what "cell culture" might be is wrong... If you start with a seed or seedling it seems that you are only stretching the seed..I somehow figured we were looking to "clone" a mature plant using parts that would not otherwise be, like growing tips or other conventional clone/propagation material. More or less stems and/or roots..I was also under the impression that we were exploiting a survival/repair response from the plant to achieve this..An ultra simplistic perspective would be if you were to be able to say quarter a plants stem length wise to expose the Parenchyma, assuming its undifferentiated cells inside then influence it to spring up a column of miniature clones? Otherwise extract the undifferentiated cells and provoke them to make the callus that can be manipulated in that direction.If the Parenchyma of cannabis contains the undifferentiated cells cells you are after could the differentiated cells be dissolved or mechanically stripped away so that it were predominantly the undifferentiated cells being incubated? Would this provoke more successful callus formations?
You're way ahead of me there buddy ! I dont experiment much, not a mad scientist either.Short answer is....I have no idea,but heres what I do I take a cutting spec from a new tip, midway down. It grows callus, gets bigger. then I cut a piece off,transfer,and it grows roots. I transfer the roots, and they shoot up a bonzai tree quickly. I harden, then put them in the veg system. thats the extent of what I do today. I keep jars of "unusable roots" waiting for my order. anything done over the years was just figuring out what not to do, then directions came out to do this. I followed directions. on the cheap. with success. only issue is sterilizing plant material . the rest is second nature now.Only taking up a plant count number at the last moment when told to shoot, this allows me to have rooting genetics on standby, negating the seedling/cloning phase. these "seedlings" have a ball of roots that rival a month old plant....all before they even sprout !
Once a farm is established of chosen root ball genetics, and regular transfers take place, it really is a no brainer. lots of back ups in case of failures, common sense, years on the internet, or a week in a book. they're only super plants for a couple weeks, then look quite normal, except for the excessive lateral branching, ie lots of roots in the beginning.
many strains will grow from seed directly into flower(seed from 12/12) with minimal harvest weight loss. if i experiment any, it will be with moving a bonzai from a jar and putting directly in the flower room. other than that, I have no plans to exploit the technology any further than my own applications. lots of success can be achieved in vitro with a good rooting gel, on the cheap, if needed. varying amounts do really wild things in culture.
I culture plant material in different stages, so that I can micropropagate them to fulfill my needs. I hope that helps
crafted by the big pros. I may never bother with any other again. those 5 in my stash all the time have proven to be pure bliss for every occasion leaving me wanting for nothing, but another rip !!
