you actually have to sterilize the water by boiling it for 10 minutes first. sterilize the syringes too by sucking the hot water up into them, letting it sit for a bit, ejecting. do that a few times for each syringe, the last time you do it, let the water cool for at least 2 minutes before mixing it with your spores. i like to scrape them off the print into the glass while I'm waiting for the water to cool. you also have to let the water/spore solution sit for at least 12 hours before inocculating the substrate.