Tissue Culture (New age Cloning )

DCgrow505

Well-Known Member
Whats going on my fellow Green Grower's . So I have been doing a bit of research on TC cloning and it really sounds cool and i can't wait to try it . So what the hell is tissue cloning and why should I care . At its called micro cloning because true TC cloning is done in a lab and is really tedious . Micro cloning is sort of like normal cloning but a lot more sterile and on a miniature scale in jars. Its quickly becoming the way that most clones are made and genetics are kept . Which is why you should care (as a example almost all of the plants a home depot are TC .Think limitless number of plants that are all identical but without the huge mother rooms and problems that were all used to .
The process in a nutshell is this Take small tip cuttings from a mother plant and wash them thoroughly with soap and water then alcohol the diluted bleach without roots as a micro bonsai. . Whats special gelatin . It come in TC kits Official Its purified water standard nutrient salts , vitamins and amino acids, branching hormone, preservative, and gellan gum to make it all gelatinous.. Every five weeks or so, the cultures woo grow from one node to approximately five and then are cut apart and put into five new jars . This is the multiplication stage repeat as necessary until you have enough new moms to supply your needs. Whenever you need clones, you cut it into five parts as before , then put them in a jar with rooting hormones instead of branching hormones and wait two weeks . At that point its time to move them to a standard clone tray with grow medium and a dome to let them plump up a bit from one inch to more like five . You now have a whole tray of beautiful clones that will grow into large productive plants.
Now Here's some of the pro's of (TC)
Keeps 100 strains on a shelf under a single T5 and you only have to re-jar every five to eight weeks .
Watering is no longer needed...........Crazy right
Genetic drift is eliminated , and even damaged genetics can be brought bavk to their original vigor .
The wattage used for mothers and clones is greatly reduced.
Bugs and mold are no longer part of the process.
There is no other practical way to provide high numbers of clones (thousands at a time)that all short with a high node count and at the same growth stage .
Which is perfect for a sea of green setup or rotating gardens.

Now HERE'S THE CONS
Tot have to plan five weeks ahead,versus two for traditional cloning .
There's a 10 week initial lead time to deal with.
It's a cleanly process,so if you have an aversion to being clean, may have some problems.
There are places out there that supply all of this stuff in kit form .One is pure food Gardening , which you can find at planttc.com
Now there you have it its probly the coolest and best way to take clones . Now has any one done this or have one of the kits to do it .Cause im going to be getting one and would like to hear any tips or coments about it and im going to be documenting it and updating this thread so we can how it works out .Cause i have a fuck load of strains and really don't have the room for mothers any more and this way i will be able to keep all my good genetics cause lets face its hard to let go of a damn good strain just cause you got a new one that you want to try but dont have the room for a other mother so its ether one or the other . Now i can not only keep all my strain but i can start getting more and just store them and start them back up when ever i feel like it . Sound's like its worth a try shit . What do you guys think and it
 

DankBudzzz

Well-Known Member
You're going to quickly realize that without lab conditions and different antibodies, gibberellins etc, results will be hard to impossible to come by... The generic kits from the store may work but usually in tissue culturing you have to adapt your media and hormone levels numerous times to see results.
 

Jack Harer

Well-Known Member
I've been reading up on this as well. It looks promising. You do not need "sterile lab conditions", just a clean place to do the work, and the proper equipment to do so. Anyone who can grow shrooms can probably do this.
 

DCgrow505

Well-Known Member
Yeah i agree with jack harer as long as your not a pig and dirty as fuck it can be done . Sure Im not going to get it right the fist time but just like any thing with time im sure i can pull this off . I use to grow mushrooms a long time ago and found that to be pretty easy . So stay tuned cause if i try something i do it to the best of my ability . But if any body has done or has any tips lets hear it
 

Springtucky

Well-Known Member
I'm with Cannawizard. Sub'd definitely something I would consider...I currently have 13 mothers and would love to be able to get rid of some of them with the option of bringing them back for future runs and still having downsized mother area.
 

Banditt

Well-Known Member
been interested in this ever since I read about it in high times a while back. I don't have room for mothers and this would really allow me to scale down the amount of room I need to keep mothers. but I'd like to see how it works out for other people before I spring the 250 or whatever it is for the kit.
 

Jack Harer

Well-Known Member
I wonder if you can do this from frozen plant tissue. THAT would make it worth the effort. If not, then the second benefit I can see may come into play. Taking clones in flower. If you can take mid/late flower clones without having to re-veg them, that would be a huge bonus as well.
If frozen tissue can't be done, then there is still the need for fresh tissue. Hopefully, late flower clones would be possible.
 
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