water cure?
- Thread starter savant
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budbomb
Active Member
Hey, water curing is when you soak your bud in water before drying it out. The idea is to "wash away" some of the plant matter leaving a 'purer' bud. You lose a bit of mass but also a lot of the odour and taste making it good for stealth smoking, i suppose it depends what you like
more info:
https://www.rollitup.org/harvesting-curing/102336-comparing-air-water-cure-w.html
more info:
https://www.rollitup.org/harvesting-curing/102336-comparing-air-water-cure-w.html
there is just nothing like the dank smell of your own excellent weed,,,, lovin it......
Seamaiden
Well-Known Member
Look up GreenPhoenix's thread on it. I did pretty much according to that thread, except I didn't punch holes in my Mason jars (I'd like to reuse them). You'll lose a lot more of the weight, I lost about 80% and another fellow I know lost 84% of the weight. This, along with the loss of scent, odor, and taste, can be a good thing or a bad thing, depending on your needs and preferences.
bmarley043
Well-Known Member
yeah, my last plant i harvested, i split it up and water cured 1/4 of it and air cured 3/4 of it. i did 7 days and it came out with nice packed buds, and was dufinitely potent
It was more like the water when you've got a vase of cut flowers (smelled like that, too).
RhymesWithGosh
Well-Known Member
Here is a link to a step by step guide to water cure.
https://www.rollitup.org/harvesting-curing/102641-greenx-how-tos-how-water.html
https://www.rollitup.org/harvesting-curing/102641-greenx-how-tos-how-water.html
budbomb
Active Member
I'm really sorry but i'm going to have to correct you there shepj, chlorophyll is an extremely hydrophobic membrane bound molecule and therefore is completely insoluble.
By water curing you allow the reductive degredatation of the membranes surounding intracellular organelles. This releases their contents allowing enzymatic breakdown of much of the cellular material. In this way the thylakoid membrane is breached allowing convertion of chlorophyll into nonfluorescent chlorophyll catabolites (which are colourless). Depending on how long the bud is left underwater for these can then leech out of the cells into solution.
Really sorry to be such a panickity cock! No offense intended!
By water curing you allow the reductive degredatation of the membranes surounding intracellular organelles. This releases their contents allowing enzymatic breakdown of much of the cellular material. In this way the thylakoid membrane is breached allowing convertion of chlorophyll into nonfluorescent chlorophyll catabolites (which are colourless). Depending on how long the bud is left underwater for these can then leech out of the cells into solution.
Really sorry to be such a panickity cock! No offense intended!
grandpabear3
New Member
that shit just made my eyes and ears bleed.that was like reading the matrix or sum shit. wow, someone is entirely too smart not to be an evil supervillan of sorts....i'll be watching you.
i think you would like bigbudballs he's got a brain on him too.
budbomb
Active Member
Lol! I like this evil supervillan idea! Apologies for the turbo science, am doing a PhD in biochemistry so it all sort of comes out when i get baked!
You're definitely right on the impurities front though shepj, especially things like any fertilizers not removed by flushing
You're definitely right on the impurities front though shepj, especially things like any fertilizers not removed by flushing
MrBaker
Well-Known Member
So, you're saying that in chlorophyll's case, even if it is a hydrophobe initially once it exits the thylakoids/those membranes are breached the chemistry of its new/breached environment causes a change in the cholorphyll (conformation, or otherwise) ?
I'm tryin' to fully pick up what you're puttin' down. I have a BS in bio, and I'm attemping to get into grad school for some sort of plant science (breeding/horticulture, and if not then soil science). It's so nice to converse with learned people about a substance that is so taboo here.
Seamaiden
Well-Known Member
Let it all out. This place is FRAUGHT with some of the most incredible mis-information, sometimes boggling my mind. I had no idea chlorophyll was hydrophobic! Completely, or does it have a hydrophilic end (thusly making it possibly to remove from a given water column via foam fractionation)?
Budda_Luva
Well-Known Member
ok wtf im having a hard time understanding what u guys are talkin about i have a CLUE but lemme try to explain wat i get if its not right plz corect me and explain it to me in a less educated way
Chlorophyll is hydrophobic meaning is doesnt like water right?? and is bound to the cell membrane meaning it isnt going anywhere and insoluble to water am i right???
and by this part "By water curing you allow the reductive degredatation of the membranes surounding intracellular organelles. This releases their contents allowing enzymatic breakdown of much of the cellular material." u mean by surrounding the plant matter in water u allow the breakdown of the chlorophyll thus exposing the ogranelles inside of the cell ( wat im thinkin is mostly lysosoms to break down alot of the garbage that the cell recongnizes as useless waste ) that gets rid of the usuless plant material around them am i right????? and by this "In this way the thylakoid membrane is breached allowing convertion of chlorophyll into nonfluorescent chlorophyll catabolites (which are colourless). Depending on how long the bud is left underwater for these can then leech out of the cells into solution." the chlorophyll changes into a different kind of colorless chlorophyll cataboties ( im guessing this word cataboties is basically saying lil peaces that are braking down) and by a miracle of GOD (LOL) the COLORLESS chlorophyll can leech out thru the membrane because they have no color??? that part confuses me or is it because its simply braking down matter that is of no use to the cell anymore and the cell wants to get rid of it??? is that right??
Chlorophyll is hydrophobic meaning is doesnt like water right?? and is bound to the cell membrane meaning it isnt going anywhere and insoluble to water am i right???
and by this part "By water curing you allow the reductive degredatation of the membranes surounding intracellular organelles. This releases their contents allowing enzymatic breakdown of much of the cellular material." u mean by surrounding the plant matter in water u allow the breakdown of the chlorophyll thus exposing the ogranelles inside of the cell ( wat im thinkin is mostly lysosoms to break down alot of the garbage that the cell recongnizes as useless waste ) that gets rid of the usuless plant material around them am i right????? and by this "In this way the thylakoid membrane is breached allowing convertion of chlorophyll into nonfluorescent chlorophyll catabolites (which are colourless). Depending on how long the bud is left underwater for these can then leech out of the cells into solution." the chlorophyll changes into a different kind of colorless chlorophyll cataboties ( im guessing this word cataboties is basically saying lil peaces that are braking down) and by a miracle of GOD (LOL) the COLORLESS chlorophyll can leech out thru the membrane because they have no color??? that part confuses me or is it because its simply braking down matter that is of no use to the cell anymore and the cell wants to get rid of it??? is that right??
budbomb
Active Member
The image attached is from a paper few years back, it gives a good over view of what's going on. The key steps for the solubility of the molecule are as follows:
(1) The cleavage of the long aliphatic group to form chlorophyllide. By removing this large hydrophobic group you increase solubility by...
Ok, going to have to go back to first principles here. When you solvate a molecule you pack water around it in an ordered fashion. Water doesn't like being ordered as is represents a decrease in entropy (chaos, essentially the random nature of a system), and this takes energy to occur. The reason so many things are soluble in water is because water is a polar molecule (it has slight possive and negative charges on it) which means it can interact with charged substances (eg the +ive Na and -ive Cl in salt) and also with other polar molecules, where highly electronegative elements such as O or N have drawn -ve charge towards themselves (eg the OH groups on sugar). In the later case these interactions are known as Hydrogen bonds, as the slightly +ive Hs on the water can interact. All these interactions are energetically favourable therefore can counteract the un-energetically favourable decrease in entropy. Bit hard to explain without diagrams so if anyone cares i'll sketch some out.
Ok, so back to the cleavage of the aliphatic group (by the by, aliphatic referes to linear hydrocarbon chains, as opposed to aromatic groups, which are rings, like those found on THC
). With a bit of luck you'll now see how removing a large group with no charged or polar nature is good for the solubility of the whole.(2) Next up is the removal of the chelated Mg2+ ion from the centre of the tetrapyrrol (tetra = 4, pyrrol = this particular type of 5 carbon ring) structure. Usually we like metal ions in terms of solubility, they have a nice strong +ive charge for the negative ends of H2O to cluster around. However, in this case the Mg2+ is tightly held in the centre of the ring so not too much water can get to it, and it is also hogging 4 Nitrogen atoms which are extremely good H bond acceptors. The Mg is thus removed, and water can get into the gap, resulting in lots more lovely energetically favourable bonding.
(3) Finally you get to the breaking open of the tetrapyrol structure. This opens up 3 more hydrogen bonding sites finally over-coming the entropy energy barrier and alowing the break down product to drift off out of your bud....
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budbomb
Active Member
ah shit, was going to go into the whole transport pathway bit but am late for meeting some friends so i'll have to make it quick, basically when chlorophyll is broken down naturally in the cell it is transported out of the chloroplast by atp mediated active transport, ie it takes energy. It is then transported to the vacuule. On the way it comes into contact with a whole cocktail of cytosolic enzymes that do a lot of the breakdown process. Once the cell is dead (but not dehyrated due the the underwater part) the membranes start to break down so there's no stopping the chlorophyll being degraded in situ