canndo
Well-Known Member
Perhaps Wolverine, this process will enable us to send exact genetics through the mail. Wouldn't that be wonderful. I did a complete scan of my starts this morning. I took another loss - it looks like bacteria. If I don't put an end to my other endeavor it will have an ever growing effect on this one. I consider the micropropagation much more important.
Ok, so the seeds seem to have moved, just barely so I can't call the experiment failed.
These are bottom shots so that you can see the bacterial infection, they are a bit blurry but you can see a sort of cream/yellow smear around the base of the plant. The biocide can get overwhelmed and when it does this is the result. Of course I have yet to salvage a plantlet from a contaminated jar. I believe I could do it if I use the biocide straight on the plantlet but the plantlets aren't worth the cost to me.
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Now look at the particulars of this contamination. The hairs are briliant white, they grow roughly parallel to each other and they have not yet exhibited spores or coloration. This may be a genuine first - one psychonautical organism eating another.
as I said, bacterial contamination. I don't want anyone to be discouraged - this stuff happens and it is easily controled. Once you get started, the worst thing about contamination is the bother of cleaning the jars. So long as you catch it early, you don't even have to re-sterilize the contaminated units. It is just dissapointing because as I said, I like all of them and I hate to see any wasted. We should be getting back to normal in just a few more days when I halt the other project.
This is my mistake. I chemicaly "cooked" this plantlet with alcohol, bleach, more bleach, more alcohol and I had it in the unltrasonic cleaner for a long time. It just never made it - I really wanted to see this one take off too.
This is one from that same branch - note the thick stem, that worked. I can't put all pictures of failure on here, I have to put a little sucess on this post. This plantlet was taken last week and it shows the characteristic first shoot growth, remember that I cannot put cuttings in that actually have leaves. The leaves are all but impossible to disinfect and then because the tissue is so thin, the leaves are killed. Contrary to popular belief, working with leaves of C. Sativa currently gets us nowhere. You can create callus from leaves but organogenisis - making undifferentiated cells into shoots is exceedingly difficult. Furthermore, callus tends to impart uncertain genetics to the plants that are spawned from it. Part of what I would call a "break through" is the acceptance that there needs to be no callus phase in the proceedure. Callus works for some plants but until someone comes up with a better way to shoot from callus, it doesn't work very well here.
Ok, so the seeds seem to have moved, just barely so I can't call the experiment failed.
These are bottom shots so that you can see the bacterial infection, they are a bit blurry but you can see a sort of cream/yellow smear around the base of the plant. The biocide can get overwhelmed and when it does this is the result. Of course I have yet to salvage a plantlet from a contaminated jar. I believe I could do it if I use the biocide straight on the plantlet but the plantlets aren't worth the cost to me..
Now look at the particulars of this contamination. The hairs are briliant white, they grow roughly parallel to each other and they have not yet exhibited spores or coloration. This may be a genuine first - one psychonautical organism eating another.as I said, bacterial contamination. I don't want anyone to be discouraged - this stuff happens and it is easily controled. Once you get started, the worst thing about contamination is the bother of cleaning the jars. So long as you catch it early, you don't even have to re-sterilize the contaminated units. It is just dissapointing because as I said, I like all of them and I hate to see any wasted. We should be getting back to normal in just a few more days when I halt the other project. This is my mistake. I chemicaly "cooked" this plantlet with alcohol, bleach, more bleach, more alcohol and I had it in the unltrasonic cleaner for a long time. It just never made it - I really wanted to see this one take off too. This is one from that same branch - note the thick stem, that worked. I can't put all pictures of failure on here, I have to put a little sucess on this post. This plantlet was taken last week and it shows the characteristic first shoot growth, remember that I cannot put cuttings in that actually have leaves. The leaves are all but impossible to disinfect and then because the tissue is so thin, the leaves are killed. Contrary to popular belief, working with leaves of C. Sativa currently gets us nowhere. You can create callus from leaves but organogenisis - making undifferentiated cells into shoots is exceedingly difficult. Furthermore, callus tends to impart uncertain genetics to the plants that are spawned from it. Part of what I would call a "break through" is the acceptance that there needs to be no callus phase in the proceedure. Callus works for some plants but until someone comes up with a better way to shoot from callus, it doesn't work very well here.
