My first class with the golden teacher

polyarcturus

polyarcturus

Well-Known Member
best to extract from fruits is what i hear, mycleium can be iffy since it doesnt really start to produce the compounds we want till then.


as for your ,mycelium growth. the temp swings are fine, it will slow growth as you said due to the average temps being lower. but IMO this is a good thing, 12 hours for your mycelium to grow rigorously as well as contams, and 12 hours for the mycelium the dominant organism in the container, to chill as well as the other organisms giving your mycelium a higher chance to resist contam. plus it is conducive with nature so i approve.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
My first batch I don't know about it, no sign of life nothing, nada. So I think I'll wait till tue. and if nothing happens till then I'll be throwing them jars into garbage.

I also will be knocking up rest of my four jars that I got left today at night.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
dont throw the jars away....just the contents of the jars :) i really find it hard to believe that none of your jars from spores101 got anything.....i mean i know your not lying just seems weird...

at least you got something going now its easy street.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
RetiredMatthebrute said:
dont throw the jars away....just the contents of the jars :) i really find it hard to believe that none of your jars from spores101 got anything.....i mean i know your not lying just seems weird...

at least you got something going now its easy street.
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Yeah man, none of the jars got any life in them, I'll still keep them till tue. if nothing by then their going bye bye.

You think it's safe to use the same jars? I mean I'll washed them fuckers good and shit but still you think it's a good idea? Because I was planning on throwing them fuckers out, jars and all.
 
canndo

canndo

Well-Known Member
Throw them out jar a and all - if some jars from the same syringe grew and some didn't, then you most likely have some contamination in the jar and you just can't see it.
 
Thundercat

Thundercat

Well-Known Member
Now that you guys are talking about it it might have been spores101 I had the issue with too. I ordered up about 6 3lb bags of steralized rye berries to try them out. They were in sealed filter patch grow bags. I had 3 strains I think, and shot each of them into 2 bags, and got zero growth from any of them. It was a huge waste I had been 100% more successful with my wild bird seed and homemade syringes. I can't remember where I bought the rye from but it looked good when it arrived so I can't say for sure what the issue was but like I've said before in this thread my syringes were to clear for my taste.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
canndo said:
Throw them out jar a and all - if some jars from the same syringe grew and some didn't, then you most likely have some contamination in the jar and you just can't see it.
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Yes sir if you say so, actually there's no sign of any life even contams. maybe they're there but can't just see it.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
Thundercat said:
Now that you guys are talking about it it might have been spores101 I had the issue with too. I ordered up about 6 3lb bags of steralized rye berries to try them out. They were in sealed filter patch grow bags. I had 3 strains I think, and shot each of them into 2 bags, and got zero growth from any of them. It was a huge waste I had been 100% more successful with my wild bird seed and homemade syringes. I can't remember where I bought the rye from but it looked good when it arrived so I can't say for sure what the issue was but like I've said before in this thread my syringes were to clear for my taste.
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Thunder, I think it was me. My syringes looked like plain water even took a closer look to see if anything is floating around but I didn't see anything, but that was my first time looking at a spore syringe so I thought maybe it just should looks like that. I'm not bashing spores101, Matt had luck with them so maybe it was just me or my syringes.

But when I got my syringe from sporeworks, I was like WoW I could actually see big ass clumps floating, and already got mycelium growing in two of my jars that I knocked up on march 4th.
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
did you make sure to shake up the syringe before inoculating? i have 3 jars that arent doing shit either and 9 that are doing well. i think maybe the 3 that are not doing shit are from the second syringe that i forgot to shake up....
 
Thundercat

Thundercat

Well-Known Member
Thats all I'm saying acid is I like seeing spores in my syringes. I know they can be there either way, but mentally its just nice seeing them.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
RetiredMatthebrute said:
did you make sure to shake up the syringe before inoculating? i have 3 jars that arent doing shit either and 9 that are doing well. i think maybe the 3 that are not doing shit are from the second syringe that i forgot to shake up....
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Matt, I shook the shit out of it haha
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
Thundercat said:
Thats all I'm saying acid is I like seeing spores in my syringes. I know they can be there either way, but mentally its just nice seeing them.
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Hahah Thunder exactly I like to see things to believe it's there, I mean I know spores are microscopic and shit but still if I see it I know it's there ;)
 
RetiredMatthebrute

RetiredMatthebrute

Well-Known Member
Thundercat said:
Thats all I'm saying acid is I like seeing spores in my syringes. I know they can be there either way, but mentally its just nice seeing them.
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i agree 100% here, its nice to see that you got what you ordered. i prob wont order from spores 101 again...infact im hoping to never have to order from anywhere again...going to try my hand at g2g soon and keep it rolling like that untill i can afford to get some agar (just because i think its cool to watch) and will prob be trying to spore print and make my own couple of syringes. though i would like to try to grow that penis envy strain as i have heard its extremely potent.
 
canndo

canndo

Well-Known Member
technical dan said:
I got a couple tubs for an incubator and its sitting on top of my cab with the water at 77 deg F and the inside sitting right around 74 deg F so that should speed things up a bit. The lights in the cab are on for 18 hours a day right now but I think I am going to start a 12/12 solo cup run in there once I move the current seedling out. Will the dark period and subsequent cooling/ inconsistent temp of incubation chamber negatively affect the mycelium or would it just be a bit slower due to the average temp being lower?

Canndo, poly do either of you (or anyone else) have experience with ethanol extracts? I have been reading various methods and procedures for doing the extraction some from mushrooms/ fruit bodies and one by Adam Gottlieb (1976) where he used LC and then extracted from the growth in the culture rather than fruiting. Could I fruit and then extract from the mycelium or would the vast majority of the psilocybin and psilocin have been transferred into the fruits? Any idea whether this be an effective, impossible, or impractical?

If interested there are a few procedures posted here: http://forums.lycaeum.org/index.php?topic=26844.0 including parts of Adam Gottlieb which can be goggled for the complete text.
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You can extract from the mycelium and it will contain what you are after but you will also be left with what comes over from your grain. Gottlieb latched on to some old industrialized methods for making penicilin and extrapolated from that. The proceedure is crude and certainly not worth the effort when you can simply go another few steps and have fruit. That was back when only a few had the secret of Maria Sabina's Gold.
 
canndo

canndo

Well-Known Member
aCiDjEsUs said:
Yes sir if you say so, actually there's no sign of any life even contams. maybe they're there but can't just see it.
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take one outside, open it just a bit and get a sniff, if it smells like rotten apples then you are contaminated with a very common bacteria that is not easy to see and changes the PH of the substrate to the point where your mycelium will not germinate.
 
canndo

canndo

Well-Known Member
aCiDjEsUs said:
Hahah Thunder exactly I like to see things to believe it's there, I mean I know spores are microscopic and shit but still if I see it I know it's there ;)
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If you want to get good at this you are going to have to be able to work without seeing the things you are battling or working with. Learn to see the unseeable, sense the microbes all around you - learn to understand and see the dark side of life, the decomposers and you will be a far better grower when you do.
 
aCiDjEsUs

aCiDjEsUs

Well-Known Member
canndo said:
take one outside, open it just a bit and get a sniff, if it smells like rotten apples then you are contaminated with a very common bacteria that is not easy to see and changes the PH of the substrate to the point where your mycelium will not germinate.
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I forgot to say that first batch of my jars that are not showing any sign of life are sitting on a shelf at constant 68F. But I'll take one outside and see what's up. Thank you Canndo.
 
technical dan

technical dan

Active Member
Nice growth acid.

Thanks poly.

So after fruiting could I steep/ sit the myc. in water, filter, evaporate, and then wash with ethanol? I imagine there would still be things from the grain that are soluble in both water and ethanol but is that bad other than being less pure end product than other methods? Would this be a reasonable way to get a dose-able solution?

I want do the ethanol extraction on fruits/ stones, it seems to be a great way to measure doses and to reduce and store material. I also just want to see the pure compound since I can pretty easily; it'll be fun like making hash oil. Hooray for extractions!
 
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