perennial cannabis

A glowing house plant would be hard not to buy..

Pharma, from what I have gathered and my multiple failed attempts at micro prop (some attempts went on to live longer than others), would it be possible to just accidentally manipulate something such as leaf structure? As my attempting micro prop continue, I always look back and think man I should have cleaned everything better but now im wondering if those earlier tries would have pulled through could the plant have sprouted into something containing thc but looking more alien? I mean accidentally contaminate, change the leaf, and bam grow that shit in flower beds all over the country.

wristychronicles said:

Pharma, from what I have gathered and my multiple failed attempts at micro prop (some attempts went on to live longer than others), would it be possible to just accidentally manipulate something such as leaf structure? As my attempting micro prop continue, I always look back and think man I should have cleaned everything better but now im wondering if those earlier tries would have pulled through could the plant have sprouted into something containing thc but looking more alien? I mean accidentally contaminate, change the leaf, and bam grow that shit in flower beds all over the country.

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UV light is key for this,and drugs, synthetic hormones,other plant constituents(colchicine) in vitro, and not a leaf. cut the eraser size tip top of a cutting, brand new growth, called meristem culturing. sterilize it, cut off and shave off the dead stuff when finished, seal it up. There are a few ways to do that, and we often see these samll changes express themselves as three leaf clones or similar. these are recovering damaged dna, and can be caused by a few different techniques. there are a couple different bacteria that are used to "trojan horse" the coding of the marijuana plant. We call this Bio Jacking, or GeneJack. basically a(jelly fish glow) gene is cultured on a (safe ecoli) bacteria until dividing like crazy, the to get it into the mj callus an antibiotic is used to kill the ecoli, then rinsed, then cultured with the magic other(agrobacterium) cultured, and "inserted" "naturally" in the mj plant, where it will genejack the plant, multiply(glo gene) and express. I played for years with the firefly glow because it was easier to get/work with, but could only flouresce in an a calcium rich plant...started thinking about blinking plants when they need something, or plants producing their own lights..stoner stuff, anyway. found the isolated jelly gene, ...and "got this buddy to explore some things he had no idea were patented until approached, educated, and stopped". scared, I only use non patented(natural) culture techniques now. and think my buddy does the same. The baddest ass thing I learned how to do was to breed in vitro with homemade artificial seeds, using an over the counter medicine. literally two genetic codes of two mj plants are joined, not grafted, and share genetics, so in a couple months a plant can be f1'd and stabalized, and also bred for specific traits, in months, not years.

the agro is found in nature, if you know what to look for. theres a pic of one on an mj plant, just sayin. its the exact location of an infection that "naturally" took place, I swear ! plus, it was a buddy's, and he moved to australia too.

I didn't mean by using a leaf cutting. I meant using the most viable mj plant tissues, can I manipulate the shape of the fan leaf ? If the result was a different shape it would be unrecognizable.

ahh, google roses with thc

wristychronicles said:

I know people breed for taste flavor, height, weight. I want to breed for a cold hardy perennial plant. Has anyone tried this? I'm going to unbury my outdoor girls after harvest and dig up the crown of the plant and a smaller cut of her root mass, bag all of it and place it in the fridge, to keep it fresh from drying out. I will try to reveg next spring or maybe even indoors a couple weeks later. Im am doing this with a handful of strains. IF ANY of the plant crowns stay green enough in the fridge I might have a shot. Then of course the breeding begins. Am I crazy or has this been done? the obvious goal it to eventually get a plant that can winter over with some mulch on the crown outdoors and then wakes it self up in the spring with out help from me, aside from some possible mild root trimming. It would be awesome to plant them and then never plant again. It also might take me 15 years to accomplish lol. but shit as long as im not dead ill have time. peace guys, im off to work.

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I love this idea, but as far as I understand putting the crown in a refrigerator would do nothing other than slow the rate of respiration and keep the tissues slightly more viable for a few weeks maybe months, cannabis lacks a vernalization requirement so it would not be stimulated by an extended cold period. Also, comments others have made about not having leaves or stems to regrow from, well that is irrelevant, cannabis contains dormant buds below the surface which can be made to emerge using auxin and cytokinin type hormones and many of them will just naturally emerge from which new stems can arise, but if you went through enough strains (I'm sure it would take hundreds of strains and thousands of phenos) you might be able to find one which reliably survives using the methods you mentioned, its a great idea!

There are some cannabis plants that wee breed to be perennials or they just happen to be that way. I have seen 1 or 2 of them on the attitude. I think Doggies Nuts offers some genetics that happens to be perrenials.

Da Almighty Jew said:

There are some cannabis plants that wee breed to be perennials or they just happen to be that way. I have seen 1 or 2 of them on the attitude. I think Doggies Nuts offers some genetics that happens to be perrenials.

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read the actual description. they say "The cannabis seeds and not so commonly the plant can get over winter and re-emerge come the spring, as in this little, or should we say big beauty will auto flower once it is established - if left to be, it will set cannabis seeds, then these cannabis seeds will grow and then these plants will do the same thing so on and so forth ad infinitum!"

https://www.cannabis-seeds-bank.co.uk/the-doggies-nuts-seeds-big-bad-john/prod_3154.html

it sounds to me that they've just found a really precocious ruderalis characteristic plant and bred it with higher potency strains and its really just the seeds that make it "perennial" they do say that its not common but is possible the plants will be perennial but they're so damned expensive I don't know of any detailed experiments to confirm it

Old thread but apparently this has been done, all for the low price of $200,000 from bc seeds LMFAO! It's called "forever buds".

wristychronicles,
Have you tried grafting? I have heard and read some things of what you are attempting having been done by grafting and re-grafting two or more plants back into themselves to provide the genetics you want. It may take a few generations to find a stable strain, but isn't that your ultimate goal? a stable strain capable of tolerating your weather?
You graft and reg-raft the the plants back into themselves multiple times seeking your most desirable traits taking clones and seeing how they perform. The strains you want the most genes out of you obviously re-graft in more than the others.
Eventually you make a branch that has the genes you want, clone it and you make a mother plant and make more clones and later, if you like, seeds.

Yeah, im about to post a video on grafting. The last years crown rotted out overt Winter,so im running some other cold Hardy cultivars outdoor this year the grafting i mainly do on indoor mother plants.one root stock 8 flavors

It wouldn't be perennial but more ever green if i get a strain that pull through a mild winter

It would be awsome if you could make it grow like a tree, flowering each year. But sadly I think it's gonna be a challenge!

If they are going to do genetic work with cannabis to improve it, it would be smarter to just make a tch producing mint plant or somethign that comes back and spreads with insane vigor and hardiness with a killer growth rate. It wouldn't even be cannabis at that point, just mint that makes certain cannabinoids to get you high. However, those 'perennial' seed producers on attitude look cool... Do regular canabis seeds die in cold temps? They survive in the fridge according to articles so how cold can they get before dying? IF they can take low freezing temps then they might as well be sold as that kind of "perrenial" too cuz if you let them drop seed it will survive the winter and grow in the spring.

Moontoc said:

wristychronicles,
Have you tried grafting? I have heard and read some things of what you are attempting having been done by grafting and re-grafting two or more plants back into themselves to provide the genetics you want. It may take a few generations to find a stable strain, but isn't that your ultimate goal? a stable strain capable of tolerating your weather?
You graft and reg-raft the the plants back into themselves multiple times seeking your most desirable traits taking clones and seeing how they perform. The strains you want the most genes out of you obviously re-graft in more than the others.
Eventually you make a branch that has the genes you want, clone it and you make a mother plant and make more clones and later, if you like, seeds.

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Unfortunately grafting will not alter the genetic codes of the plant no matter how many times performed. It will result in an original set code plant with different stems growing from it. When it dies it will not come back grafted again, the seeds will only correspond with the exact genetics of the branch grafted. Think of all the organ recipients eventually turning into their original donor bodies again? I used to graft cup winner bouquets for moms, to lighten the plant count. I sold them all, and culture/clone now.