wristychronicles
Well-Known Member
A glowing house plant would be hard not to buy..
UV light is key for this,and drugs, synthetic hormones,other plant constituents(colchicine) in vitro, and not a leaf. cut the eraser size tip top of a cutting, brand new growth, called meristem culturing. sterilize it, cut off and shave off the dead stuff when finished, seal it up. There are a few ways to do that, and we often see these samll changes express themselves as three leaf clones or similar. these are recovering damaged dna, and can be caused by a few different techniques. there are a couple different bacteria that are used to "trojan horse" the coding of the marijuana plant. We call this Bio Jacking, or GeneJack. basically a(jelly fish glow) gene is cultured on a (safe ecoli) bacteria until dividing like crazy, the to get it into the mj callus an antibiotic is used to kill the ecoli, then rinsed, then cultured with the magic other(agrobacterium) cultured, and "inserted" "naturally" in the mj plant, where it will genejack the plant, multiply(glo gene) and express. I played for years with the firefly glow because it was easier to get/work with, but could only flouresce in an a calcium rich plant...started thinking about blinking plants when they need something, or plants producing their own lights..stoner stuff, anyway. found the isolated jelly gene, ...and "got this buddy to explore some things he had no idea were patented until approached, educated, and stopped". scared, I only use non patented(natural) culture techniques now. and think my buddy does the same. The baddest ass thing I learned how to do was to breed in vitro with homemade artificial seeds, using an over the counter medicine. literally two genetic codes of two mj plants are joined, not grafted, and share genetics, so in a couple months a plant can be f1'd and stabalized, and also bred for specific traits, in months, not years.Pharma, from what I have gathered and my multiple failed attempts at micro prop (some attempts went on to live longer than others), would it be possible to just accidentally manipulate something such as leaf structure? As my attempting micro prop continue, I always look back and think man I should have cleaned everything better but now im wondering if those earlier tries would have pulled through could the plant have sprouted into something containing thc but looking more alien? I mean accidentally contaminate, change the leaf, and bam grow that shit in flower beds all over the country.
I love this idea, but as far as I understand putting the crown in a refrigerator would do nothing other than slow the rate of respiration and keep the tissues slightly more viable for a few weeks maybe months, cannabis lacks a vernalization requirement so it would not be stimulated by an extended cold period. Also, comments others have made about not having leaves or stems to regrow from, well that is irrelevant, cannabis contains dormant buds below the surface which can be made to emerge using auxin and cytokinin type hormones and many of them will just naturally emerge from which new stems can arise, but if you went through enough strains (I'm sure it would take hundreds of strains and thousands of phenos) you might be able to find one which reliably survives using the methods you mentioned, its a great idea!I know people breed for taste flavor, height, weight. I want to breed for a cold hardy perennial plant. Has anyone tried this? I'm going to unbury my outdoor girls after harvest and dig up the crown of the plant and a smaller cut of her root mass, bag all of it and place it in the fridge, to keep it fresh from drying out. I will try to reveg next spring or maybe even indoors a couple weeks later. Im am doing this with a handful of strains. IF ANY of the plant crowns stay green enough in the fridge I might have a shot. Then of course the breeding begins. Am I crazy or has this been done? the obvious goal it to eventually get a plant that can winter over with some mulch on the crown outdoors and then wakes it self up in the spring with out help from me, aside from some possible mild root trimming. It would be awesome to plant them and then never plant again. It also might take me 15 years to accomplish lol. but shit as long as im not dead ill have time. peace guys, im off to work.
read the actual description. they say "The cannabis seeds and not so commonly the plant can get over winter and re-emerge come the spring, as in this little, or should we say big beauty will auto flower once it is established - if left to be, it will set cannabis seeds, then these cannabis seeds will grow and then these plants will do the same thing so on and so forth ad infinitum!"There are some cannabis plants that wee breed to be perennials or they just happen to be that way. I have seen 1 or 2 of them on the attitude. I think Doggies Nuts offers some genetics that happens to be perrenials.
Unfortunately grafting will not alter the genetic codes of the plant no matter how many times performed. It will result in an original set code plant with different stems growing from it. When it dies it will not come back grafted again, the seeds will only correspond with the exact genetics of the branch grafted. Think of all the organ recipients eventually turning into their original donor bodies again? I used to graft cup winner bouquets for moms, to lighten the plant count. I sold them all, and culture/clone now.wristychronicles,
Have you tried grafting? I have heard and read some things of what you are attempting having been done by grafting and re-grafting two or more plants back into themselves to provide the genetics you want. It may take a few generations to find a stable strain, but isn't that your ultimate goal? a stable strain capable of tolerating your weather?
You graft and reg-raft the the plants back into themselves multiple times seeking your most desirable traits taking clones and seeing how they perform. The strains you want the most genes out of you obviously re-graft in more than the others.
Eventually you make a branch that has the genes you want, clone it and you make a mother plant and make more clones and later, if you like, seeds.