Tissue Culture/Micro Propagation Grow from Scratch

I'm still working on getting a refigerator for heating issues. It is about 85 here already and it isn't even noon. I am worried about how others will deal with heat issues but they may not be so adamant about not using their airconditioner. If I can though, after I have put this thing together I will try to display "plans" for this thing. I figure a wine cooler would be the best over all. It seems that wine cooling systems can be set at about 58 highest. It is interesting that I am chasing someone else around the web who is looking for a way to keep something between 64 and 77.

"We are looking not to maintain wine, but another item at a specific temperature range. This material must be maintained at "room temperature" (64-77 degrees Farenheit), and a normal refrigerator is too cold and the temperature range cannot really be controlled. We are concerned especially during the summer where the ambient temperature may go above 77 degrees."

It is funny that others are claiming that 58 is about as high as any cooler should get.

I never thought I would have to be going back this many steps when I started but this has become more of a passion than I first intended.

OK, I've got the fridge coming Wed. I bought another 3.6 cubic foot because it is on sale but I don't think it is large enough and it has to accomodate lights (I'm not at the LED stage yet). The temperature is getting above 90 most days and everything I have done will only change that by about 5 degrees. This is my only way of continuing this project through the summer. I figure this is a part of the whole here so I will include pictures of the modification and we can go from there. Stay with us, I didn't expect when I started that I would be doing electrics but who knows where else this might take us.

I dont see how its commercially viable so.. thanks anyway.

On the concept of commercial viability. I think it depends. Given the fact that the micropropagation industry is thriving and many millions of starts are distributed every year, at least in theory, this is viable. Many aquatic plants are distributed this way as are orchids and carniverous plants. I am curious as to why you might think this. Now I am still unsure if it is viable for the ordinary grower and I am certain for the vast majority it is certainly a series of steps one needn't take. After all, if you do a couple of dozen clones a grow, why go through all this trouble - that being said, I am quite certain that anyone who wishes to maintain a library of clones, anyone who wishes to distribute genetics and anyone who would like to mass produce a certain genetic would embrace this method. I've been around this sort of thing for some time and I do remember hearing that HIDs would never work well. More currently I still see people saying that LEDs won't work and we know this is only a matter of time. In the next few months however, we will certainly see if it is, and if the lay person can do reasonably well. It is going to be more difficult in regions like my own but that is only because I refuse to run my AC in order to cool one or two racks of jars.

I just got back from a "tour" of humbolt. Met some fascinating people, a group of growers who are very interested in these methods and damn if they didn't have gobs of wonderful smoke everywhere I went, everyone I met. Some of them took me for the best fishing trip of my life where I caught some huge salmon. So now it is back to work. As I said, I got the referigerator, I've looked at some plans and now I am going out to get some tools with which to work this thing out. There is far more interest in this stuff than I originaly thought and fewer and fewer people are claiming that it can' be done.

Fun read lots of great info love the list of supplies ... great job subbed +rep any updates are interesting from you cannado

Thanks Joe. I noticed something interesting. I follow all of the tissue culture threads and found that someone was visiting another thread (my initial one on this site actually) and they were wondering why no one was doing this. I don't think it is possible that people just never find this journal. Or maybe it bores them.


The refrigerator project is going poorly as the thermostat is not 12 volts in the thing - it is 24 and I am having a hard time finding a suitable new thermostat for it. I have, however located a new source of raw material - so there's that.

Just found this thread, great read. I have been interested in this for some time, but have not taken the first step. Ive read a couple of books and done some research ,While doing research I came across this.

http://shop.billberryfarmstissueculture.com/BBF-Plant-Tissue-Culture-Set_c12.htm

Does this look like a good starting point or would I be better going with the 'kitchen kit' ive seen every where.

What ever happend with all this canndo? you got a million plants in jars by now?

No, We are on hold until I get an incubator set up - it is consistantly over 100 degrees in my lab and nothing will live.

Nothing new to report - it is just too fucking hot to even work on things - jaysus, and humid as well.

Thank you for making this endeavor public on this forum. I am very interested in this subject and look forward to you succeeding in rooting!

I bit the bullet. I still intend to work somethig out with my refrigerator but it just doesn't seem to get my attetion like the project itself. I have air conditioned a room and am checking out consistancy and of course how much it will cost. I've had the room going for a week now and just aquired some new cuttings hopefully my chemicals aren't too stale - I suppose I should mix up a new batch but I am cranking it up again. For other reasons,I intend to keep the room between 80 and 84 degrees and am now doubting that this range will work for our experiments - we will see though. I will inlud pictres,I believe you will be shocked at the size of the new layout. I now can acommodate up to 500 jars and have 300 ready to go - if I have enough cuttings that is.

Fantastic thread! Thank you so much for sharing your experiences in tissue culture. I have recently taken my first plantlets and your thread helped tremendously. Keep it up!!!!

Jah love.

It is getting cooler, I took a large batch of cuttigs in anticipaition of making a new report but they all failed. I believe I need to remake all of my solutions. The conversion of the fridge was a stunning failure. This is not a dead project.

maybe not dead but surely more death than life contained therein

I have not give up on you. When you crack the code let us know and post pics. Good luck on your next run.

It wont work.

it can be dead! I just started my first batch!

budforever442200 said:

it can be dead! I just started my first batch!View attachment 1793284

Click to expand...

Let me know when you have a bud. Till then its sprout that just wont grow new tops. That's where everyone before you has failed. Now please dont take my comments negatively, I hope someone makes a success. Yours isnt yet, its just where everyone else left off after failing.