What did you accomplish today?

UncleBuck

Well-Known Member
I might use 2x4s. I have a bunch of little pieces laying around, not a lot of 2x2. My 1x4s are 10ft for roofing so I'm not sure if I want to cut those up.
2x4 for cleats is code for a very good reason

4 screws through each cleat

you should have inside stringers 12'' OC too
 

tyler.durden

Well-Known Member
Another harvest week, very busy. After a decade, I think I'm going to have to retire my Trainwreck strain. It's only producing half of what it used to. When I started growing about 10 years ago (it's weird to write that, that's a while) the current data suggested that cloned plants are identical to its parent. But current data is suggesting the opposite - Current Science Cloning Article

Out of about 15 or so phenos of strains that I've kept and cloned for commercial production, this is only the second one that has accumulated net negative characteristics through the mutations inherent in cloning. The other strain I lost was a gorgeous cut of Northern Lights #5. Everyone mourned that death. I started this TW at the same time as an OG Kush mom that, if anything, has actually gotten better through time (except that it has become a little more difficult to clone.) I have these fast flowering Durban fem seeds that I've yet to pop, if I find a good pheno I think this will replace the TW. Oh well. ♫ Nothing lasts forever, even cold November Rain ♫


Making new moms, so I took a bunch of extra cuts and filled the cloner before culling the old -

IMG_1538.JPG


New moms. Sorry about the fucked up lines...

IMG_1534.JPG

Planted these on 4/20, so they should turn out to be Super Moms...
 

curious2garden

Well-Known Mod
Staff member
Another harvest week, very busy. After a decade, I think I'm going to have to retire my Trainwreck strain. It's only producing half of what it used to. When I started growing about 10 years ago (it's weird to write that, that's a while) the current data suggested that cloned plants are identical to its parent. But current data is suggesting the opposite - Current Science Cloning Article

Out of about 15 or so phenos of strains that I've kept and cloned for commercial production, this is only the second one that has accumulated net negative characteristics through the mutations inherent in cloning. The other strain I lost was a gorgeous cut of Northern Lights #5. Everyone mourned that death. I started this TW at the same time as an OG Kush mom that, if anything, has actually gotten better through time (except that it has become a little more difficult to clone.) I have these fast flowering Durban fem seeds that I've yet to pop, if I find a good pheno I think this will replace the TW. Oh well. ♫ Nothing lasts forever, even cold November Rain ♫


Making new moms, so I took a bunch of extra cuts and filled the cloner before culling the old -

View attachment 4323131


New moms. Sorry about the fucked up lines...

View attachment 4323134

Planted these on 4/20, so they should turn out to be Super Moms...
I've heard that tissue culture will clean up 'issues' with older clone generation. I honestly don't know but it's something to consider.
 

tyler.durden

Well-Known Member
I've heard that tissue culture will clean up 'issues' with older clone generation. I honestly don't know but it's something to consider.
If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy ;)
 

curious2garden

Well-Known Mod
Staff member
If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy ;)
I admire how you name the fallacy you do not wish to fall foul of LOL Your mind always amazes me.

I'm etoh impaired as this is Wednesday night. I'll answer you when I'm sober. My OCD compels me.
 

Singlemalt

Well-Known Member
Another harvest week, very busy. After a decade, I think I'm going to have to retire my Trainwreck strain. It's only producing half of what it used to. When I started growing about 10 years ago (it's weird to write that, that's a while) the current data suggested that cloned plants are identical to its parent. But current data is suggesting the opposite - Current Science Cloning Article

Out of about 15 or so phenos of strains that I've kept and cloned for commercial production, this is only the second one that has accumulated net negative characteristics through the mutations inherent in cloning. The other strain I lost was a gorgeous cut of Northern Lights #5. Everyone mourned that death. I started this TW at the same time as an OG Kush mom that, if anything, has actually gotten better through time (except that it has become a little more difficult to clone.) I have these fast flowering Durban fem seeds that I've yet to pop, if I find a good pheno I think this will replace the TW. Oh well. ♫ Nothing lasts forever, even cold November Rain ♫


Making new moms, so I took a bunch of extra cuts and filled the cloner before culling the old -

View attachment 4323131


New moms. Sorry about the fucked up lines...

View attachment 4323134

Planted these on 4/20, so they should turn out to be Super Moms...
Excellent set-up, kudos. Clean and professional, just like some research labs I've seen.
 

Singlemalt

Well-Known Member
If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy ;)
The cannabis genome isn't that well mapped out yet; one would need to know the specific sequence causing the negative phenotype and the specific sequence of the desired trait(s) before clipping. Arabidopsis is an old, well known and respected experimental system in botany, much like Drosophila is in genetics wherein the genome is well mapped. We aren't there yet with Cannabis
 

curious2garden

Well-Known Mod
Staff member
The cannabis genome isn't that well mapped out yet; one would need to know the specific sequence causing the negative phenotype and the specific sequence of the desired trait(s) before clipping. Arabidopsis is an old, well known and respected experimental system in botany, much like Drosophila is in genetics wherein the genome is well mapped. We aren't there yet with Cannabis
My understanding is a meristem tissue culture is virus free. If causation is genetic drift over time then you need whole sequence genomics. That was my impaired line of thought.

If you could point me to some helpful links, I'd appreciate it. I'm no botanist, but I can't fathom how tissue culture can correct net negative mutations of a phenotype. Gotta look into it, though. I don't want to commit an argument from ignorance fallacy ;)
To expensive and not realistic yet, there are companies out there: http://www.segra-intl.com/cannabis-tissue-culture-production/# so someday.
 

BudmanTX

Well-Known Member
Morning everyone.....

it's a nice 59F and clear this morning, mid 80's by the afternoon.....

there is way to much science talk for my understand without coffee right now....working on the ol first cup right now......

welp off to the ck the garden and the tomatoes ;) to see what things look like after yesterdays storms....

looks like this weekend i'm gonna pull out the ol experiment gonna be nice and sunny for the next couple of day it looks like.....gonna have to do some rednecking with some string with it.....yippee....

later off to the shop gotta do some stock orders at least get them written up for the suppliers...
 
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